Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of California, San Diego, California 92161, USA.
Otol Neurotol. 2011 Jan;32(1):158-62. doi: 10.1097/MAO.0b013e3181feb92a.
We hypothesize that vestibular schwannomas (VSs) exhibit up-regulation of estrogen receptor (ER) at the protein level compared with control great auricular nerve (GAN).
It has been reported in the literature that VS occur more commonly in women and tend to be larger and more vascular in women, and growth rate can accelerate during pregnancy. The literature contains widely divergent results on ER expression in VS, however, varying from no detectable levels to detection of ER in all samples.
Sixteen sporadic VS specimens were immediately snap-frozen after microsurgical excision and analyzed for phosphorylated and total levels of ERα with Western blot analysis. ERα expression levels were normalized to actin; then, relative expression to GAN was determined.
All VS specimens exhibited expression of both phosphorylated and total ERα. Total ERα expression in VS is equivalent to or slightly up-regulated compared with GAN. VS specimens exhibited more pronounced up-regulation of phosphorylated (i.e., activated) levels of ERα compared with GAN.
We have demonstrated that ERα expression in VS is equivalent to GAN. The phosphorylated form of the receptor is up-regulated compared with GAN, however, indicating a higher level of ERα activation in sporadic VS compared with normal nerve. Further investigation into antiestrogen therapy for VS is warranted.
我们假设前庭神经鞘瘤（VSs）在蛋白质水平上表现出雌激素受体（ER）的上调，与对照的耳大神经（GAN）相比。
文献报道 VS 在女性中更常见，并且在女性中往往更大、更具血管性，并且在怀孕期间生长速度可以加快。然而，关于 ER 在 VS 中的表达，文献中存在广泛不同的结果，从无法检测到的水平到所有样本中均检测到 ER。
16 例散发性 VS 标本在显微切除后立即冷冻，并通过 Western blot 分析分析磷酸化和总 ERα水平。将 ERα 表达水平标准化为肌动蛋白；然后，确定相对于 GAN 的相对表达。
所有 VS 标本均表达磷酸化和总 ERα。与 GAN 相比，VS 中的总 ERα表达水平相等或略有上调。与 GAN 相比，VS 标本中 ERα 的磷酸化（即激活）水平上调更为明显。
我们已经证明 ERα 在 VS 中的表达与 GAN 相当。然而，受体的磷酸化形式与 GAN 相比上调，表明散发性 VS 中 ERα 的激活水平高于正常神经。进一步研究针对 VS 的抗雌激素治疗是合理的。
