Kajiwara K, Ito H, Fukumoto T
Department of Neurosurgery, Yamaguchi University School of Medicine, Japan.
J Neuroimmunol. 1990 May;27(2-3):133-40. doi: 10.1016/0165-5728(90)90062-r.
An investigation was conducted to determine whether lymphocytes were able to infiltrate into the brain parenchyma of normal rats in which hyperosmotic opening of the blood-brain barrier (BBB) had been created by internal carotid infusion of filtered 20% mannitol. Various numbers (1 x 10(6) to 3 x 10(8] of autologous lymphocytes were injected into the internal carotid artery, or via a tail vein, after BBB opening. Lymphocytes were detectable in the brain of rats subjected to high-grade BBB opening and injected with a high number of lymphocytes. Subsets of lymphocytes were examined by immunohistochemical staining using OX19, W3/25 and OX8 monoclonal antibodies. There was no remarkable difference in number between W3/25- and OX8-positive cells. From these results it was suggested that the intensity of BBB opening and the number of lymphocytes flowing in brain vessels are related to the passage of lymphocytes through the BBB.
进行了一项研究，以确定淋巴细胞是否能够浸润到正常大鼠的脑实质中，这些大鼠通过颈内动脉输注经滤过的20%甘露醇建立了血脑屏障（BBB）的高渗开放。在BBB开放后，将不同数量（1×10⁶至3×10⁸）的自体淋巴细胞注入颈内动脉或经尾静脉注射。在经历高级别BBB开放并注射大量淋巴细胞的大鼠脑中可检测到淋巴细胞。使用OX19、W3/25和OX8单克隆抗体通过免疫组织化学染色检查淋巴细胞亚群。W3/25阳性细胞和OX8阳性细胞的数量没有显著差异。从这些结果表明，BBB开放的强度和流入脑血管的淋巴细胞数量与淋巴细胞通过BBB的过程有关。
