一种用于检测β-半乳糖苷酶融合蛋白的异质性双抗体酶联免疫测定法。
A heterogeneous double antibody enzyme-linked immunoassay to measure beta-galactosidase fusion protein.
作者信息
Hayashibe K, Sassano D, Ziai M R
机构信息
Department of Dermatology, Kobe University, Japan.
出版信息
J Immunoassay. 1990;11(1):89-95. doi: 10.1080/01971529008053260.
A rapid and sensitive enzyme-linked immunoassay (ELISA) to quantitate recombinant fusion proteins encoded by cloned cDNA in the bacteriophage lambda gt11 is described. Since the fusion protein is expressed in an equimolar ratio to beta-galactosidase, the assay derives the concentration of the recombinant protein in total bacterial lysates or pure preparations from the measurement of beta-galactosidase with an enzyme-linked immunoassay. This assay is a useful technique to measure the recombinant proteins for subsequent immunological and biochemical characterization.
本文描述了一种快速灵敏的酶联免疫吸附测定（ELISA）方法，用于定量噬菌体λgt11中克隆的cDNA编码的重组融合蛋白。由于融合蛋白与β-半乳糖苷酶以等摩尔比表达，该测定法通过酶联免疫吸附测定法测量β-半乳糖苷酶，从而得出总细菌裂解物或纯制剂中重组蛋白的浓度。该测定法是一种用于测量重组蛋白以便进行后续免疫学和生化特性鉴定的有用技术。
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