Seillan C
Laboratoire des Sciences de la Consommation, INRA-CRJ, Jouy en Josas, France.
Lipids. 1990 Mar;25(3):172-6. doi: 10.1007/BF02544334.
We studied the actions of oxysterols on fatty acid distribution and lipid synthesis in cultured bovine aortic smooth muscle cells. Cultures were labeled with [1-14C]arachidonate or [1-14C]oleate. During a 24-hr incubation, 25- or 22R-hydroxycholesterol enhanced the incorporation of label into triglycerides, concomitant with a reduction in the labeling of phospholipids. Cholestantriol or 20-hydroxycholesterol had the opposite effects. They caused a higher incorporation of radiolabel into phospholipids and a reduction of labeling of triacylglycerols. Similar changes were seen in cells labeled with [1-14C]acetate. Therefore, we conclude that oxysterols can promote changes in the distribution of fatty acids between neutral lipids and phospholipids through mechanisms that still need to be clarified.
我们研究了氧化甾醇对培养的牛主动脉平滑肌细胞中脂肪酸分布和脂质合成的作用。培养物用[1-¹⁴C]花生四烯酸或[1-¹⁴C]油酸进行标记。在24小时的孵育过程中，25-羟基胆固醇或22R-羟基胆固醇增强了标记物掺入甘油三酯的过程，同时伴随着磷脂标记的减少。胆甾三醇或20-羟基胆固醇则有相反的作用。它们导致放射性标记物更多地掺入磷脂，并减少了三酰甘油的标记。在用[1-¹⁴C]乙酸盐标记的细胞中也观察到了类似的变化。因此，我们得出结论，氧化甾醇可通过仍需阐明的机制促进中性脂质和磷脂之间脂肪酸分布的变化。
