Cousins D V, Francis B R, Gow B L, Collins D M, McGlashan C H, Gregory A, Mackenzie R M
Animal Health Laboratories, Department of Agriculture, Baron-Hay Court, South Perth, Western Australia.
Res Vet Sci. 1990 Mar;48(2):196-200.
Culture of tuberculous lesions from six of 14 captive seals yielded an organism which, on the basis of biochemical and drug sensitivity tests, was identified as Mycobacterium bovis, although the organism showed a weak cording pattern and was glycerol tolerant. It was pathogenic in rabbits and guinea pigs and after passage the organism exhibited strong cord formation and was glycerol intolerant. Restriction endonuclease analysis and sodium dodecyl-sulphate polyacrylamide gel electrophoresis indicated that the organism belonged to the Mycobacterium tuberculosis complex. Restriction patterns indicated that infection in the six seals was from a single source. Western blotting with monoclonal antibody to M bovis identified antigens at 23 and 27 kDa in M bovis which were absent from the seal isolates.
从14只圈养海豹中的6只身上获取的结核病变培养物中分离出一种微生物。基于生化和药敏试验，该微生物被鉴定为牛分枝杆菌，尽管它呈现出较弱的索状形态且耐受甘油。它对兔子和豚鼠具有致病性，传代后该微生物呈现出强烈的索状形成且不耐受甘油。限制性内切酶分析和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳表明该微生物属于结核分枝杆菌复合群。限制性图谱表明这6只海豹的感染来自单一来源。用抗牛分枝杆菌单克隆抗体进行的蛋白质印迹法在牛分枝杆菌中鉴定出了23 kDa和27 kDa的抗原，而海豹分离株中不存在这些抗原。
