Rodríguez J G, Fissanoti J C, Del Portillo P, Patarroyo M E, Romano M I, Cataldi A
Corporación CorpoGen, Hospital San Juan de Dios, Santafé de Bogotá, D.C. Colombia.
J Clin Microbiol. 1999 Jul;37(7):2330-2. doi: 10.1128/JCM.37.7.2330-2332.1999.
The presence of a 500-bp fragment which amplifies a region from the genome of Mycobacterium bovis (J. G. Rodriguez, G. A. Meija, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131-2138, 1995) was evaluated by carrying out PCR on 121 M. bovis isolates. The M. bovis strains, previously characterized by culture and biochemical tests, were isolated from cattle in different regions of Argentina, Mexico, and Colombia. Four additional strains isolated from sea lions that belong to the M. tuberculosis complex were also included in the study. All of the isolates tested were PCR positive, rendering the expected 500-bp band and giving a correlation of 100% with previous microbiological characterization. Southern blot analysis revealed a common band of 1, 800 bp and a polymorphic high-molecular-mass hybridization pattern. The results show that this assay may be useful for diagnosis and identification of M. bovis in cattle.
通过对121株牛分枝杆菌分离株进行聚合酶链反应（PCR），评估了一个500碱基对片段的存在情况，该片段可扩增牛分枝杆菌基因组中的一个区域（J.G.罗德里格斯、G.A.梅亚、P.德尔波蒂略、M.E.帕塔罗约和L.A.穆里略，《微生物学》141:2131 - 2138，1995年）。这些牛分枝杆菌菌株先前已通过培养和生化试验进行了鉴定，它们是从阿根廷、墨西哥和哥伦比亚不同地区的牛身上分离得到的。该研究还包括另外4株从属于结核分枝杆菌复合群的海狮身上分离得到的菌株。所有检测的分离株PCR均呈阳性，产生了预期的500碱基对条带，并且与先前的微生物学鉴定的相关性为100%。Southern印迹分析显示出一条1800碱基对的共同条带和一种多态性的高分子量杂交模式。结果表明，该检测方法可能有助于牛分枝杆菌在牛群中的诊断和鉴定。
