Lundin K E, Sollid L M, Bosnes V, Gaudernack G, Thorsby E
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Scand J Immunol. 1990 Apr;31(4):469-75. doi: 10.1111/j.1365-3083.1990.tb02794.x.
HLA class II molecules may be induced on non-lymphoid cells by gamma-interferon (IFN-gamma). We investigated if HLA class II molecules induced by IFN-gamma on the HT29 colonic carcinoma cell line are functional, i.e. if they may be recognized by allogeneic T cells. We found that IFN-gamma-treated HT29 (HT29IFN) cells could not induce primary proliferative responses of peripheral blood T lymphocytes, nor were they able to induce proliferation in T-lymphocyte clones (TLC) specific for HLA class II molecules found on HT29IFN. However, in the presence of exogenous interleukin 2 (IL-2), 1 of 5 DQw8-specific TLC proliferated when restimulated with HT29IFN, and 3 of these 5 TLC could very effectively inhibit the growth of HT29IFN, probably due to a cytotoxic effect. Both the proliferative response and the cytotoxicity were inhibited by anti-DQ MoAb. We conclude that T cells may recognize HLA-DQ molecules on non-lymphoid cells, which may be of relevance for autoimmune diseases, graft-versus-host disease, and possibly for the recognition of malignant cells.
γ干扰素（IFN-γ）可诱导非淋巴细胞表达Ⅱ类人白细胞抗原（HLA）分子。我们研究了IFN-γ诱导HT29结肠癌细胞系表达的HLAⅡ类分子是否具有功能，即它们是否能被同种异体T细胞识别。我们发现，经IFN-γ处理的HT29（HT29IFN）细胞不能诱导外周血T淋巴细胞的原发性增殖反应，也不能诱导对HT29IFN上发现的Ⅱ类HLA分子特异的T淋巴细胞克隆（TLC）增殖。然而，在外源性白细胞介素2（IL-2）存在的情况下，5个DQw8特异性TLC中有1个在用HT29IFN再次刺激时发生增殖，并且这5个TLC中的3个能够非常有效地抑制HT29IFN的生长，这可能是由于细胞毒性作用。增殖反应和细胞毒性均被抗-DQ单克隆抗体抑制。我们得出结论，T细胞可能识别非淋巴细胞上的HLA-DQ分子，这可能与自身免疫性疾病、移植物抗宿主病以及可能对恶性细胞的识别有关。
