Department of Agricultural Chemistry, Kyoto University, Kitashirakawa, Oiwake-cho, Sakyo-ku, Kyoto 606, Japan.
Appl Environ Microbiol. 1988 Jul;54(7):1766-9. doi: 10.1128/aem.54.7.1766-1769.1988.
Nitrile hydratase, which occurs abundantly in cells of Rhodococcus rhodochrous J1 isolated from soil samples, catalyzes the hydration of 3-cyanopyridine to nicotinamide. By using resting cells, the reaction conditions for nicotinamide production were optimized. Under the optimum conditions, 100% of the added 12 M 3-cyanopyridine was converted to nicotinamide without the formation of nicotinic acid, and the highest yield achieved was 1,465 g of nicotinamide per liter of reaction mixture containing resting cells (1.48 g as dry cell weight) in 9 h. The nicotinamide produced was crystallized and then identified physicochemically. The further conversion of the nicotinamide to nicotinic acid was due to the low activity of nicotinamide as a substrate for the amidase(s) present in this organism.
腈水合酶大量存在于从土壤样本中分离出的红球菌属(Rhodococcus rhodochrous J1)细胞中，可催化 3-氰基吡啶的水合作用生成烟酰胺。利用休止细胞，优化了生产烟酰胺的反应条件。在最佳条件下，添加的 12 M 3-氰基吡啶 100%转化为烟酰胺，而没有形成烟酸，在 9 小时内，每升含有休止细胞（1.48 克干细胞重量）的反应混合物中获得的最高产率为 1,465 克烟酰胺。生成的烟酰胺被结晶，然后进行物理化学鉴定。由于酰胺酶（存在于该生物中的）对烟酰胺作为底物的活性低，导致烟酰胺进一步转化为烟酸。
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