College of Agriculture and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Sci China Life Sci. 2010 Sep;53(9):1112-22. doi: 10.1007/s11427-010-4060-y. Epub 2010 Nov 23.
Previous reports indicated that AtMYB103 has an important role in tapetum development, callose dissolution, and exine formation in A. thaliana anthers. Here, we further characterized its function in anther development by expression pattern analysis, transmission electron microscopy observation of the knockout mutant, and microarray analysis of downstream genes. A total of 818 genes differentially expressed between ms188 and the wild-type were identified by global expression profiling analysis. Functional classification showed that loss-of-function of AtMYB103 impairs cell wall modification, lipid metabolic pathways, and signal transduction throughout anther development. RNA in situ hybridization confirmed that transcription factors acting downstream of AtMYB103 (At1g06280 and At1g02040) were expressed in the tapetum and microspores at later stages, suggesting that they might have important roles in microsporogenesis. These results indicated that AtMYB103 is a crucial regulator of Arabidopsis anther development.
先前的报告表明，AtMYB103 在拟南芥花药的绒毡层发育、胼胝质溶解和花粉外壁形成中起着重要作用。在这里，我们通过表达模式分析、敲除突变体的透射电子显微镜观察以及下游基因的微阵列分析，进一步研究了其在花药发育中的功能。通过全局表达谱分析，共鉴定出 ms188 与野生型之间差异表达的 818 个基因。功能分类表明，AtMYB103 的功能丧失会损害花药发育过程中的细胞壁修饰、脂质代谢途径和信号转导。RNA 原位杂交证实，AtMYB103 下游的转录因子（At1g06280 和 At1g02040）在后期的绒毡层和小孢子中表达，表明它们可能在小孢子发生中具有重要作用。这些结果表明，AtMYB103 是拟南芥花药发育的关键调节因子。
