College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, 830052, China.
College of Agriculture and Hydraulic Engineering, Sui Hua University, No.18, Huanghe Road, Suihua, 152061, China.
Sci Rep. 2021 Feb 22;11(1):4330. doi: 10.1038/s41598-021-83912-8.
Silicon plays a vital role in plant growth. However, molecular mechanisms in response to silicon have not previously been studied in wheat. In this study, we used RNA-seq technology to identify differentially expressed genes (DEGs) in wheat seedlings treated with silicon. Results showed that many wheat genes responded to silicon treatment, including 3057 DEGs, of which 6.25% (191/3057) were predicted transcription factors (TFs). Approximately 14.67% (28 out of 191) of the differentially expressed TFs belonged to the MYB TF family. Gene ontology (GO) enrichment showed that the highly enriched DEGs were responsible for secondary biosynthetic processes. According to KEGG pathway analysis, the DEGs were related to chaperones and folding catalysts, phenylpropanoid biosynthesis, and protein processing in the endoplasmic reticulum. Moreover, 411 R2R3-MYB TFs were identified in the wheat genome, all of which were classified into 15 groups and accordingly named S1-S15. Among them, 28 were down-regulated under silicon treatment. This study revealed the essential role of MYB TFs in the silicon response mechanism of plants, and provides important genetic resources for breeding silicon-tolerant wheat.
硅在植物生长中起着至关重要的作用。然而，以前尚未在小麦中研究过对硅的分子机制响应。在这项研究中，我们使用 RNA-seq 技术来鉴定硅处理的小麦幼苗中的差异表达基因（DEGs）。结果表明，许多小麦基因对硅处理有反应，包括 3057 个 DEGs，其中 6.25%（191/3057）被预测为转录因子（TFs）。大约 14.67%（28 个/191 个）的差异表达 TFs 属于 MYB TF 家族。基因本体（GO）富集显示，高度富集的 DEGs 负责次生生物合成过程。根据 KEGG 途径分析，DEGs 与伴侣和折叠催化剂、苯丙烷生物合成以及内质网中的蛋白质加工有关。此外，在小麦基因组中鉴定出 411 个 R2R3-MYB TFs，它们均分为 15 组，并分别命名为 S1-S15。其中，28 个在硅处理下下调。本研究揭示了 MYB TFs 在植物硅响应机制中的重要作用，并为培育硅耐受小麦提供了重要的遗传资源。
