Reid W A, Branch T, Gorman C, Kay J
Department of Pathology, University of Leeds, U.K.
J Pathol. 1990 Mar;160(3):203-7. doi: 10.1002/path.1711600304.
The aspartic proteinase zymogen, progastricsin, which occurs in normal gastroduodenal mucosa and prostate, has been localized by the immunogold-silver method in formalin-fixed, paraffin-embedded sections of metastatic adenocarcinoma in lymph nodes and liver, the primary site being known in each case. Progastricsin was demonstrated in 16 of 84 lymph node metastases, including 7 of 17 from stomach, 2 of 2 from prostate, and 7 of 65 from other sites. Progastricsin was also found in 19 of 98 hepatic metastases, including 8 of 22 from stomach, 6 of 24 from pancreas, and 5 of 52 from other sites. The presence of progastricsin in a metastasis correlated well with a primary tumour in the stomach or prostate or, less significantly, pancreas. Immunolocalization of progastricsin in a histological section of metastatic adenocarcinoma may help to locate the primary site.
天冬氨酸蛋白酶原（即前胃蛋白酶）存在于正常胃十二指肠黏膜和前列腺中，运用免疫金银法在淋巴结和肝脏转移性腺癌的福尔马林固定、石蜡包埋切片中对其进行了定位，每种情况下的原发部位均已知晓。在前胃蛋白酶的定位中，84例淋巴结转移中有16例显示有该酶，其中包括17例来自胃部转移中的7例、2例来自前列腺转移中的2例以及65例来自其他部位转移中的7例。在98例肝转移中也发现了19例前胃蛋白酶，其中包括22例来自胃部转移中的8例、24例来自胰腺转移中的6例以及52例来自其他部位转移中的5例。转移灶中前胃蛋白酶的存在与胃或前列腺原发性肿瘤密切相关，与胰腺原发性肿瘤的相关性则较弱。在转移性腺癌组织切片中对前胃蛋白酶进行免疫定位，可能有助于确定原发部位。
