Ogura H
Gan. 1977 Aug;68(4):423-6.
The possibility of clong avian tumor virus (ATV) by the transfection technique was studied. The attempts required efficient facilitator for the uptake of exogenous cellular DNA by chick embryo cells (CEC) to increase transfection efficiency. The calcium method was more efficient than the DEAE-dextran method for the uptake of tritium-labeled DNA, and the calcium method was used in further segregation studies of ATV by transfection of cellular DNA from ATV-infected cells. The test viruses included the BAI-A strain of avian myeloblastosis virus (a mixture of subgroup A and B viruses) and the B77 strain of avian sarcoma virus (a mixture of transforming and transformation-defective viruses). Analysis of the recovered progeny viruses after transfection revealed that they were indeed segregates of the parent viruses.
研究了通过转染技术克隆禽肿瘤病毒（ATV）的可能性。这些尝试需要高效的促进剂来促使鸡胚细胞（CEC）摄取外源细胞DNA，以提高转染效率。在摄取氚标记的DNA方面，钙法比DEAE-葡聚糖法更有效，并且在通过转染来自ATV感染细胞的细胞DNA对ATV进行进一步分离研究中使用了钙法。测试病毒包括禽成髓细胞瘤病毒的BAI-A株（A亚群和B亚群病毒的混合物）和禽肉瘤病毒的B77株（转化病毒和转化缺陷病毒的混合物）。转染后对回收的子代病毒进行分析表明，它们确实是亲代病毒的分离株。
