Silva R F, Perbal B, Bergmann D G, Baluda M A
J Virol. 1982 Oct;44(1):422-5. doi: 10.1128/JVI.44.1.422-425.1982.
The avian myeloblastosis virus provirus inserted in a lambda bacteriophage, recombinant clone 11A1-1 (Souza et al., Proc. Natl. Acad. Sci. U.S.A. 77:3004-3008, 1980), was transfected into chicken embryo fibroblasts which had been preinfected with either Rous-associated virus type 61 or the transformation-defective avian sarcoma virus tdB77. Within 4 to 5 h after transfection, the cells were injected into 16-day-old chicken embryos or 1-day-old chicks. Acute myeloblastic leukemia developed after a long latent period. Filtered (0.22-micrometer pores) supernatant of transformed buffy-coat cell cultures from one leukemic chicken of the lambda 11A1-1 (tdB77) group rapidly transformed yolk sac cells in vitro. Results from an infectivity interference assay and analysis of proviral DNA fragments generated with restriction endonucleases were consistent with the presence in leukemic cells of defective avian myeloblastosis virus and tdB77 as the helper virus.
插入到λ噬菌体中的禽成髓细胞瘤病毒前病毒，重组克隆11A1-1（苏扎等人，《美国国家科学院院刊》77:3004 - 3008，1980年），被转染到预先感染了61型劳斯相关病毒或转化缺陷型禽肉瘤病毒tdB77的鸡胚成纤维细胞中。转染后4至5小时内，将这些细胞注射到16日龄鸡胚或1日龄雏鸡体内。经过很长的潜伏期后发生了急性髓细胞白血病。来自λ11A1-1（tdB77）组一只白血病鸡的转化后的血沉棕黄层细胞培养物的过滤（0.22微米孔径）上清液在体外能迅速转化卵黄囊细胞。感染性干扰试验结果以及用限制性内切酶产生的前病毒DNA片段分析结果与白血病细胞中存在缺陷型禽成髓细胞瘤病毒以及tdB77作为辅助病毒相一致。
