人及禽流感病毒诱导的人肺上皮细胞细胞因子和趋化因子基因表达谱。
Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses.
机构信息
Department of Microbiology, The Chinese University of Hong Kong, New Territories, Hong Kong Special Administration Region, People's Republic of China.
出版信息
Virol J. 2010 Nov 26;7:344. doi: 10.1186/1743-422X-7-344.
Abstract
Influenza pandemic remains a serious threat to human health. In this study, the repertoire of host cellular cytokine and chemokine responses to infections with highly pathogenic avian influenza H5N1, low pathogenicity avian influenza H9N2 and seasonal human influenza H1N1 were compared using an in vitro system based on human pulmonary epithelial cells. The results showed that H5N1 was more potent than H9N2 and H1N1 in inducing CXCL-10/IP-10, TNF-alpha and CCL-5/RANTES. The cytokine/chemokine profiles for H9N2, in general, resembled those of H1N1. Of interest, only H1N1, but none of the avian subtypes examined could induce a persistent elevation of the immune-regulatory cytokine - TGF-β2. The differential expression of cytokines/chemokines following infection with different influenza viruses could be a key determinant for clinical outcome. The potential of using these cytokines/chemokines as prognostic markers or targets of therapy is worth exploring.
摘要
流感大流行仍然是对人类健康的严重威胁。在这项研究中，使用基于人肺上皮细胞的体外系统比较了高致病性禽流感 H5N1、低致病性禽流感 H9N2 和季节性人流感 H1N1 感染宿主细胞细胞因子和趋化因子反应的谱。结果表明，H5N1 比 H9N2 和 H1N1 更能诱导 CXCL-10/IP-10、TNF-α和 CCL-5/RANTES。H9N2 的细胞因子/趋化因子谱总体上与 H1N1 相似。有趣的是，只有 H1N1，而不是所检查的任何一种禽流感亚型，都能诱导免疫调节细胞因子-TGF-β2 的持续升高。感染不同流感病毒后细胞因子/趋化因子的差异表达可能是临床结果的关键决定因素。这些细胞因子/趋化因子作为预后标志物或治疗靶点的潜力值得探索。
相似文献
1
Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses.
Virol J. 2010 Nov 26;7:344. doi: 10.1186/1743-422X-7-344.
2
Constitutively Expressed IFITM3 Protein in Human Endothelial Cells Poses an Early Infection Block to Human Influenza Viruses.
J Virol. 2016 Nov 28;90(24):11157-11167. doi: 10.1128/JVI.01254-16. Print 2016 Dec 15.
3
Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells.
Respir Res. 2005 Nov 11;6(1):135. doi: 10.1186/1465-9921-6-135.
4
Dynamic analysis of expression of chemokine and cytokine gene responses to H5N1 and H9N2 avian influenza viruses in DF-1 cells.
Microbiol Immunol. 2018 May;62(5):327-340. doi: 10.1111/1348-0421.12588. Epub 2018 May 4.
5
Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells.
Respir Res. 2009 Oct 30;10(1):102. doi: 10.1186/1465-9921-10-102.
6
Cellular response to influenza virus infection: a potential role for autophagy in CXCL10 and interferon-alpha induction.
Cell Mol Immunol. 2010 Jul;7(4):263-70. doi: 10.1038/cmi.2010.25. Epub 2010 May 17.
7
Differential expression of chemokines and their receptors in adult and neonatal macrophages infected with human or avian influenza viruses.
J Infect Dis. 2006 Jul 1;194(1):61-70. doi: 10.1086/504690. Epub 2006 May 26.
8
Apoptosis, cytokine and chemokine induction by non-structural 1 (NS1) proteins encoded by different influenza subtypes.
Virol J. 2011 Dec 21;8:554. doi: 10.1186/1743-422X-8-554.
9
Induction profiles of mRNA of toll like receptors and cytokines in chickens pre-exposed to low pathogenic avian influenza H9N2 virus followed by challenge with highly pathogenic avian influenza H5N1 virus.
Microb Pathog. 2018 Apr;117:200-205. doi: 10.1016/j.micpath.2018.02.041. Epub 2018 Feb 21.
10
Differential Modulation of Innate Immune Responses in Human Primary Cells by Influenza A Viruses Carrying Human or Avian Nonstructural Protein 1.
J Virol. 2019 Dec 12;94(1). doi: 10.1128/JVI.00999-19.
引用本文的文献
1
The intersection of influenza infection and autoimmunity.
Front Immunol. 2025 Apr 3;16:1558386. doi: 10.3389/fimmu.2025.1558386. eCollection 2025.
2
RNA Viruses, Toll-Like Receptors, and Cytokines: The Perfect Storm?
J Innate Immun. 2025;17(1):126-153. doi: 10.1159/000543608. Epub 2025 Jan 16.
3
Amino acid 138 in the HA of a H3N2 subtype influenza A virus increases affinity for the lower respiratory tract and alveolar macrophages in pigs.
PLoS Pathog. 2024 Feb 20;20(2):e1012026. doi: 10.1371/journal.ppat.1012026. eCollection 2024 Feb.
4
Tpl2 Ablation Leads to Hypercytokinemia and Excessive Cellular Infiltration to the Lungs During Late Stages of Influenza Infection.
Front Immunol. 2021 Oct 7;12:738490. doi: 10.3389/fimmu.2021.738490. eCollection 2021.
5
Divergent Mast Cell Responses Modulate Antiviral Immunity During Influenza Virus Infection.
Front Cell Infect Microbiol. 2021 Feb 19;11:580679. doi: 10.3389/fcimb.2021.580679. eCollection 2021.
6
Targeted Profiling of Immunological Genes during Norovirus Replication in Human Intestinal Enteroids.
Viruses. 2021 Jan 21;13(2):155. doi: 10.3390/v13020155.
7
Effect of bovine leukemia virus (BLV) infection on bovine mammary epithelial cells RNA-seq transcriptome profile.
PLoS One. 2020 Jun 24;15(6):e0234939. doi: 10.1371/journal.pone.0234939. eCollection 2020.
8
How the Respiratory Epithelium Senses and Reacts to Influenza Virus.
Am J Respir Cell Mol Biol. 2019 Mar;60(3):259-268. doi: 10.1165/rcmb.2018-0247TR.
9
The role of respiratory epithelium in host defence against influenza virus infection.
Biomed J. 2018 Aug;41(4):218-233. doi: 10.1016/j.bj.2018.08.004. Epub 2018 Sep 10.
10
Deep sequencing of primary human lung epithelial cells challenged with H5N1 influenza virus reveals a proviral role for CEACAM1.
Sci Rep. 2018 Oct 19;8(1):15468. doi: 10.1038/s41598-018-33605-6.
本文引用的文献
1
Early and sustained innate immune response defines pathology and death in nonhuman primates infected by highly pathogenic influenza virus.
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3455-60. doi: 10.1073/pnas.0813234106. Epub 2009 Feb 13.
2
The stability of mRNA influences the temporal order of the induction of genes encoding inflammatory molecules.
Nat Immunol. 2009 Mar;10(3):281-8. doi: 10.1038/ni.1699. Epub 2009 Feb 8.
3
Induction of proinflammatory cytokines in primary human macrophages by influenza A virus (H5N1) is selectively regulated by IFN regulatory factor 3 and p38 MAPK.
J Immunol. 2009 Jan 15;182(2):1088-98. doi: 10.4049/jimmunol.182.2.1088.
4
Genetic characterization of two low pathogenic avian influenza virus H5N1 isolates from Ontario, Canada.
Virus Genes. 2009 Feb;38(1):149-54. doi: 10.1007/s11262-008-0311-4. Epub 2008 Dec 12.
5
Genetic characterization and protective immunity of cold-adapted attenuated avian H9N2 influenza vaccine.
Vaccine. 2008 Dec 2;26(51):6569-76. doi: 10.1016/j.vaccine.2008.09.043.
6
Pathogenesis of emerging avian influenza viruses in mammals and the host innate immune response.
Immunol Rev. 2008 Oct;225:68-84. doi: 10.1111/j.1600-065X.2008.00690.x.
7
H5N1 transmission and disease: observations from the frontlines.
Pediatr Infect Dis J. 2008 Oct;27(10 Suppl):S54-6. doi: 10.1097/INF.0b013e3181684d2d.
8
Analysis of cytokine secretion from human plasmacytoid dendritic cells infected with H5N1 or low-pathogenicity influenza viruses.
Virology. 2008 Nov 10;381(1):22-8. doi: 10.1016/j.virol.2008.08.018. Epub 2008 Sep 17.
9
Isolation and pathotyping of H9N2 avian influenza viruses in Indian poultry.
Vet Microbiol. 2009 Jan 1;133(1-2):154-63. doi: 10.1016/j.vetmic.2008.06.013. Epub 2008 Jun 22.
10
Characterization of a pathogenic H9N2 influenza A virus isolated from central China in 2007.
Arch Virol. 2008;153(8):1549-55. doi: 10.1007/s00705-008-0139-1. Epub 2008 Jun 24.
Zotero 插件