State Key Laboratory of Microbial Technology, Shandong University, Jinan, China 250100.
Int J Food Microbiol. 2011 Jan 5;144(3):400-5. doi: 10.1016/j.ijfoodmicro.2010.10.026. Epub 2010 Oct 29.
The complete genomic sequence of Lactobacillus fermentum temperate bacteriophage ФPYB5 was determined. The phage possesses a linear, double-stranded DNA genome of 32,847 bp with a G+C content of 45.21%. A total of 46 putative open reading frames (ORFs) were identified. On the basis of homology comparisons, 25 ORFs could be assigned putative functions. The genome of bacteriophage ФPYB5 is highly modular with functionally related genes clustered together. Genome DNA of temperate bacteriophage ФPYB5, induced from heterofermentative lactic acid bacteria, showed to be closely related to that of the prophage of heterofermentative Lactobacillus reuteri 100-23 and heterofermetative Leuconostoc oenos bacteriophage 10MC in an evolutionary aspect.
已确定发酵乳杆菌温和噬菌体 ФPYB5 的完整基因组序列。噬菌体具有线性、双链 DNA 基因组，大小为 32847bp，G+C 含量为 45.21%。总共鉴定出 46 个推定开放阅读框（ORF）。根据同源性比较，可将 25 个 ORF 分配到推定功能上。噬菌体 ФPYB5 的基因组高度模块化，功能相关的基因聚集在一起。从异型发酵乳酸细菌中诱导出的温和噬菌体 ФPYB5 的基因组 DNA 在进化方面与异型发酵的乳酸乳杆菌 100-23 的噬菌体和异型发酵的肠膜明串珠菌噬菌体 10MC 的原噬菌体密切相关。
