College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-Dong, Sungbuk-Gu, Seoul, Korea.
Arch Virol. 2011 Oct;156(10):1909-12. doi: 10.1007/s00705-011-1082-0. Epub 2011 Aug 3.
Bacteriophage LF1, a newly isolated temperate phage from a mitomycin-C-induced lysate of wild type Lactobacillus fermentum, was found to contain a double-strand DNA of 42,606 base pairs (bp) with a G+C content of 45%. Bioinformatic analysis of the phage genome revealed 57 putative open reading frames (ORFs). The predicted protein products of ORFs were determined and described. According to morphological analysis by transmission electron microscopy (TEM), LF1 has an isometric head and a non-contractile tail, indicating that it belongs to the family Siphoviridae. The temperate phage LF1 has a good genetic mosaic relationship with ΦPYB5 in the packaging module. To our knowledge, this is first report of genomic sequencing and characterization of temperate phage LF1 from wild-type L. fermentum isolated from Kimchi in Korea.
从米托霉素 C 诱导的野生型发酵乳杆菌裂解物中分离到的一种新的温和噬菌体 LF1，其双链 DNA 大小为 42606 碱基对（bp），G+C 含量为 45%。噬菌体基因组的生物信息学分析显示了 57 个可能的开放阅读框（ORFs）。对 ORFs 的预测蛋白产物进行了测定和描述。根据透射电子显微镜（TEM）的形态分析，LF1 具有等径头部和不可收缩的尾部，表明它属于肌尾噬菌体科。温和噬菌体 LF1 在包装模块与 ΦPYB5 具有良好的遗传镶嵌关系。据我们所知，这是首次报道从韩国泡菜中分离到的野生型发酵乳杆菌的温和噬菌体 LF1 的基因组测序和特征描述。
