m7GMP对蛋白质-RNA相互作用的非特异性影响。
Nonspecific effect of m7GMP on protein-RNA interactions.
作者信息
Sonenberg N, Shatkin A J
出版信息
J Biol Chem. 1978 Oct 10;253(19):6630-2.
PMID:211125
Abstract
Cap analogs m7GMP and m7GDP inhibit binding of eukaryotic initiation factors to reovirus capped mRNA but also inhibit complex formation involving uncapped mRNA or 18 S rRNA. Furthermore, Escherichia coli DNA-dependent RNA polymerase binds 18 S rRNA and this interaction is also blocked by m7GMP. These results indicate that inhibition by cap analogs is not a stringent test for putative cap-specific binding between proteins and mRNA.
摘要
帽类似物m7GMP和m7GDP可抑制真核起始因子与呼肠孤病毒加帽mRNA的结合，但也会抑制涉及未加帽mRNA或18S rRNA的复合物形成。此外，大肠杆菌DNA依赖性RNA聚合酶可结合18S rRNA，这种相互作用也会被m7GMP阻断。这些结果表明，帽类似物的抑制作用并非对蛋白质与mRNA之间假定的帽特异性结合的严格测试。
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