MRC Human Reproductive Sciences Unit, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.
Mol Hum Reprod. 2011 Mar;17(3):182-92. doi: 10.1093/molehr/gaq094. Epub 2010 Nov 26.
Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system. The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle. The analysis identified PTGFR to have a distinct expression profile compared with other components of the prostanoid system, as expression is maximal during the proliferative phase. Immunohistochemical analysis for PTGER1 suggests a dual function for this receptor depending on its temporal (proliferative versus secretory) and spatial (nuclear versus cell membrane) expression. The expression profiles of the PGF(2α) synthases identified AKR1B1 and CBR1 as the likely regulators of PGF(2α) production during the menstrual phase. Immunohistochemical analysis for AKR1B1, CBR1 and AKR1C3 suggest expression to be in the glandular epithelium and vasculature. This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium. The expression profiles described have the potential to identify specific prostanoid components that may be dysregulated in inflammatory-associated disorders of the endometrium.
前列腺素是炎症过程中描述良好的主要介质，对于女性生殖系统的正常生理功能至关重要。本研究旨在确定前列腺素生物合成酶（PTGS1、PTGS2、PTGES、PTGES2、PTGES3、AKR1B1、AKR1C3、CBR1、HPGDS、PTGDS、PTGIS、TBXAS1 和 HPGD）和前列腺素受体（PTGER1、PTGER2、PTGER3、PTGER4、PTGFR、PTGDR、GPR44、PTGIR 和 TBXA2R）在整个月经周期中在人子宫内膜中的时间表达。分析表明，PTGFR 的表达模式与前列腺素系统的其他成分明显不同，因为其表达在增殖期最大。PTGER1 的免疫组织化学分析表明，该受体具有双重功能，具体取决于其时间（增殖期与分泌期）和空间（核与细胞膜）表达。鉴定的 PGF(2α)合成酶的表达谱表明 AKR1B1 和 CBR1 可能是月经期间 PGF(2α)产生的调节因子。AKR1B1、CBR1 和 AKR1C3 的免疫组织化学分析表明表达存在于腺上皮和脉管系统中。本研究代表了对人子宫内膜中前列腺素生物合成和信号通路成分的首次全面分析。描述的表达谱有可能确定在子宫内膜炎症相关疾病中可能失调的特定前列腺素成分。
