Zhu Junyan, Mayr Doris, Kuhn Christina, Mahner Sven, Jeschke Udo, von Schönfeldt Viktoria
Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Marchioninistr. 15, 81377, Munich, Germany.
Department of Gynecology and Obstetrics, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Dongfang Rd. 1630, Shanghai, 200127, China.
Histochem Cell Biol. 2018 Feb;149(2):153-160. doi: 10.1007/s00418-017-1616-y. Epub 2017 Nov 13.
Prostaglandin E2 (PGE2) is well described to be associated with both endometrial functions and disorders. The primary aim of this study was to explore the underlying mechanisms that affect the growth and function of endometrial epithelium and stroma by assessing the staining intensity of PGE2 receptors (EP) in healthy endometrium across the menstrual cycle and in pathological endometrium, such as ovarian endometriosis and endometrial cancer. We retrospectively analyzed the EPs staining intensity in human nonpregnant endometrium throughout the menstrual cycle by immunohistochemistry and further focused on EP1 (n = 42). The variation of EP1 was compared among healthy endometrium, ovarian endometriosis (n = 14), and endometrial cancer (n = 140) crosswise. EP1 presented cyclical changes with increased intensity in both epithelium and stroma during the proliferative phase. EP1 staining in the epithelium was increased in endometriotic tissue compared to healthy endometrium and tumor tissue, while in the stroma, the staining in the tumor was lower than that in both normal tissue and endometriosis. No significant differences in EP1 intensity were detected for histological, stage, grading, metastatic and recurrent subtypes in endometrial cancer. EP1 was also correlated with neither progression-free survival nor overall survival of patients with cancer. EP1 staining in progesterone receptor B (PRB)-positive tumor was stronger compared to PRB-negative tumor. EP1 may play a role in human endometrial physiology and pathology. Further studies on the effect of EP1 on human endometrium are needed.
前列腺素E2（PGE2）与子宫内膜功能及疾病均密切相关。本研究的主要目的是通过评估月经周期中健康子宫内膜以及病理状态下的子宫内膜（如卵巢子宫内膜异位症和子宫内膜癌）中PGE2受体（EP）的染色强度，来探究影响子宫内膜上皮和基质生长及功能的潜在机制。我们通过免疫组织化学回顾性分析了整个月经周期中人类非孕子宫内膜的EP染色强度，并进一步聚焦于EP1（n = 42）。横向比较了健康子宫内膜、卵巢子宫内膜异位症（n = 14）和子宫内膜癌（n = 140）中EP1的变化情况。在增殖期，EP1在上皮和基质中均呈现周期性变化，强度增加。与健康子宫内膜和肿瘤组织相比，子宫内膜异位症组织上皮中的EP1染色增加，而在基质中，肿瘤组织中的染色低于正常组织和子宫内膜异位症。在子宫内膜癌的组织学、分期、分级、转移和复发亚型中，未检测到EP1强度的显著差异。EP1也与癌症患者的无进展生存期和总生存期均无相关性。与PRB阴性肿瘤相比，PRB阳性肿瘤中的EP1染色更强。EP1可能在人类子宫内膜的生理和病理过程中发挥作用。需要进一步研究EP1对人类子宫内膜的影响。
