Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran.
Mol Biol Rep. 2011 Oct;38(7):4309-13. doi: 10.1007/s11033-010-0555-7. Epub 2010 Nov 27.
Expression pattern analysis has been revealed that glutathione S-transferase omega 2 (GSTO2, a member of class omega) is ubiquitously expressed. Over expression of GSTO2 induced apoptosis. The gene encoding GSTO2 was localized to human chromosome 10q24.3, a region that may harbor gene(s) involved in the developing of colorectal cancer. To investigate the association between GSTO2 N142D genetic polymorphism and susceptibility to colorectal cancer the present study was done. We studied 63 (26 females, 37 males) colorectal cancer patients and 126 (52 females, 74 males) healthy individuals. The control subjects were frequency matched for age and gender with the colorectal cancer group. The genotypes were performed using RFLP-PCR method. The ND and DD genotypes were not associated with risk of colorectal cancer, in comparison with the NN genotype. Family history for cancer in the first degree of relatives significantly differed between cases and controls (P = 0.012). The profiles of GSTO2 genotypes and family history in control and cancerous groups were compared to each other. Subjects with NN genotype and positive family history significantly were at high risk to develop colorectal cancer in comparison with subjects with DD or ND genotypes and negative family history (P = 0.003). Present findings indicating that GSTO2 NN genotype increase the risk of colorectal cancer in persons with positive family history for cancer in the first degree relatives.
表达模式分析表明，谷胱甘肽 S-转移酶ω 2（GSTO2，ω 类的一个成员）广泛表达。GSTO2 的过表达诱导细胞凋亡。编码 GSTO2 的基因定位于人类染色体 10q24.3，该区域可能包含参与结直肠癌发生的基因。为了研究 GSTO2 N142D 遗传多态性与结直肠癌易感性之间的关系，进行了本研究。我们研究了 63 例（26 名女性，37 名男性）结直肠癌患者和 126 名（52 名女性，74 名男性）健康个体。对照组按年龄和性别与结直肠癌组相匹配。使用 RFLP-PCR 方法进行基因型分析。与 NN 基因型相比，ND 和 DD 基因型与结直肠癌的风险无关。一级亲属的癌症家族史在病例组和对照组之间差异显著（P = 0.012）。比较了对照组和癌症组的 GSTO2 基因型和家族史特征。与 DD 或 ND 基因型和阴性家族史的个体相比，具有 NN 基因型和阳性家族史的个体发生结直肠癌的风险显著升高（P = 0.003）。本研究结果表明，具有 NN 基因型的 GSTO2 会增加一级亲属有癌症家族史的个体患结直肠癌的风险。
