Liu Jingfang, Zhao Zhenjun, Willcox Mark D P, Xu Banglao, Shi Bingyin
Department of Endocrinology, First Hospital Affiliated, Medical School of Xi'an Jiaotong University, Shaanxi, PR China.
J Immunoassay Immunochem. 2010;31(4):279-89. doi: 10.1080/15321819.2010.524860.
Renal damage of diabetic patients is linked to chronic low-grade inflammation. The aim of this study was to analyze urinary concentrations of 27 cytokines in type 2 diabetic patients with normo- and microalbuminuria by multiplex bead immunoassay. Urinary levels of IL-8, IP-10, MCP-1, G-CSF, EOTAXIN, RANTES, and TNF-α in microalbuminuric patients were significantly increased compared to those in normoalbuminuric patients (p < 0.05) or controls (p < 0.05). GM-CSF, MIP-1α, and MIP-1β levels were more elevated in microalbuminuric patients than in controls (both p < 0.05). IP-10 and MCP-1 levels were correlated with urinary albumin excretion rate (r = 0.668 and 0.544, both p < 0.01), and estimated glomerular filtration rate (r = -0.454 and -0.418, both p < 0.05). EOTAXIN, GM-CSF, IP-10, MCP-1, and RANTES levels were correlated with HbA1c (r = 0.457, 0.466, 0.678, 0.567, 0.542, respectively, p ≤ 0.01). These results indicate that urinary cytokine determination might be useful for the early diagnosis and management of patients with diabetic nephropathy.
糖尿病患者的肾损伤与慢性低度炎症有关。本研究的目的是通过多重微珠免疫测定法分析2型糖尿病正常白蛋白尿和微量白蛋白尿患者尿液中27种细胞因子的浓度。与正常白蛋白尿患者（p<0.05）或对照组（p<0.05）相比，微量白蛋白尿患者尿液中IL-8、IP-10、MCP-1、G-CSF、嗜酸性粒细胞趋化因子、RANTES和TNF-α水平显著升高。微量白蛋白尿患者的GM-CSF、MIP-1α和MIP-1β水平比对照组升高更明显（均p<0.05）。IP-10和MCP-1水平与尿白蛋白排泄率相关（r = 0.668和0.544，均p<0.01），与估计肾小球滤过率相关（r = -0.454和-0.418，均p<0.05）。嗜酸性粒细胞趋化因子、GM-CSF、IP-10、MCP-1和RANTES水平与糖化血红蛋白相关（分别为r = 0.457、0.466、0.678、0.567、0.542，p≤0.01）。这些结果表明，测定尿细胞因子可能有助于糖尿病肾病患者的早期诊断和管理。
