Discipline of General Pathology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
Department of Microbiology, Immunology and Parasitology, Institute of Biological and Natural Sciences of Federal University of Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
PLoS One. 2020 Mar 4;15(3):e0229765. doi: 10.1371/journal.pone.0229765. eCollection 2020.
To evaluate the serum concentrations of inflammatory mediators in patients with type 2 diabetes mellitus (T2DM) with or without renal alteration (RA) function.
Serum samples from 76 patients with T2DM and 24 healthy individuals were selected. Patients with T2DM were divided into two groups according to eGFR (> or < 60mL/min/1.73m2). Cytokines, chemokines and adipokines levels were evaluated using the Multiplex immunoassay and ELISA.
TNFR1 and leptin were higher in the T2DM group with RA than in the T2DM group without RA and control group. All patients with T2DM showed increased resistin, IL-8, and MIP-1α compared to the control group. Adiponectin were higher and IL-4 decreased in the T2DM group with RA compared to the control group. eGFR positively correlated with IL-4 and negatively with TNFR1, TNFR2, and leptin in patients with T2DM. In the T2DM group with RA, eGFR was negatively correlated with TNFR1 and resistin. TNFR1 was positively correlated with resistin and leptin, as well as resistin with IL-8 and leptin.
Increased levels of TNFR1, adipokines, chemokines and decrease of IL-4 play important role in the inflammatory process developed in T2DM and decreased renal function. We also suggest that TNFR1 is a strong predictor of renal dysfunction in patients with T2DM.
评估伴有或不伴有肾功能改变（RA）的 2 型糖尿病（T2DM）患者的血清炎症介质浓度。
选择 76 例 T2DM 患者和 24 例健康个体的血清样本。根据 eGFR（>60mL/min/1.73m2）将 T2DM 患者分为两组。使用 Multiplex 免疫分析和 ELISA 评估细胞因子、趋化因子和脂肪因子水平。
RA 组的 TNFR1 和瘦素高于无 RA 组和对照组。与对照组相比，所有 T2DM 患者的抵抗素、IL-8 和 MIP-1α 均升高。与对照组相比，RA 组的脂联素升高，IL-4 降低。eGFR 与 T2DM 患者的 IL-4 呈正相关，与 TNFR1、TNFR2 和瘦素呈负相关。在伴有 RA 的 T2DM 组中，eGFR 与 TNFR1 和抵抗素呈负相关。TNFR1 与抵抗素和瘦素呈正相关，抵抗素与 IL-8 和瘦素呈正相关。
TNFR1、脂肪因子、趋化因子水平升高和 IL-4 降低在 T2DM 炎症过程和肾功能下降中起重要作用。我们还表明，TNFR1 是 T2DM 患者肾功能不全的强预测因子。
