School of Biological Sciences, Flinders University of South Australia, Adelaide, GPO Box 2100, Adelaide SA 5001 Australia.
J Environ Manage. 2011 Mar;92(3):866-71. doi: 10.1016/j.jenvman.2010.10.059. Epub 2010 Nov 27.
We investigated the possibility of re-using remediated soils for new bioremediation projects by spiking these soils with waste oil sludge in laboratory based microcosms. The level of Total Petroleum Hydrocarbon (TPH) reduction was high (>80%) in naturally attenuated microcosms and was not significantly improved by biostimulation, bioaugmentation and the combined treatment of bioaugmentation and biostimulation by week 12. This indicated that the observed TPH reduction might have been related to the soil's inherent hydrocarbon-degrading potential. Microbial community analysis (16S rDNA and ITS-based Denaturing Gradient Gel Electrophoresis fingerprints) confirmed the dominance of hydrocarbon degrading genera such as Alcanivorax and Scedosporium. Cluster and Shannon diversity analysis revealed similar but stable bacterial and fungal communities in naturally attenuated and amended microcosms indicating that rapid reduction in TPH may not always be accompanied by changes in soil microbial communities. This study has therefore shown that soils previously used for bioremediation can have an improved hydrocarbon degrading potential which was successfully re-harnessed for new projects. This ability to re-harness this potential is attractive because it substantially reduces operational costs as no additional bioremediation treatments are needed. It can also extend a landfill's lifespan as soils can be re-used again before landfill disposal.
我们通过在实验室微宇宙中用废油污泥污染这些土壤，研究了再利用修复土壤用于新的生物修复项目的可能性。在自然衰减的微宇宙中，总石油烃（TPH）的减少率很高（>80%），并且通过生物刺激、生物强化和生物强化与生物刺激的联合处理在第 12 周时并没有显著提高。这表明观察到的 TPH 减少可能与土壤固有的烃类降解潜力有关。微生物群落分析（16S rDNA 和基于 ITS 的变性梯度凝胶电泳指纹图谱）证实了烃类降解属如 Alcanivorax 和 Scedosporium 的优势。聚类和 Shannon 多样性分析表明，在自然衰减和添加的微宇宙中，细菌和真菌群落相似但稳定，这表明 TPH 的快速减少并不总是伴随着土壤微生物群落的变化。因此，这项研究表明，以前用于生物修复的土壤可以提高烃类降解潜力，并且可以成功地重新用于新项目。这种重新利用这种潜力的能力很有吸引力，因为它大大降低了运营成本，因为不需要额外的生物修复处理。它还可以延长垃圾填埋场的使用寿命，因为在进行垃圾填埋处理之前可以再次使用土壤。
