Department of Biotechnology, National Institute of Technology Durgapur, Durgapur 713 209, WB, India.
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721 302, WB, India; School of Bio Science, Indian Institute of Technology Kharagpur, Kharagpur 721 302, WB, India.
Bioresour Technol. 2018 Apr;253:22-32. doi: 10.1016/j.biortech.2018.01.004. Epub 2018 Jan 3.
Scope for developing an engineered bioremediation strategy for the treatment of hydrocarbon-rich petroleum refinery waste was investigated through biostimulation and bioaugmentation approaches. Enhanced (46-55%) total petroleum hydrocarbon (TPH) attenuation was achieved through phosphate, nitrate or nitrate+phosphate amendment in the sludge with increased (upto 12%) abundance of fermentative, hydrocarbon degrading, sulfate-reducing, CO-assimilating and methanogenic microorganisms (Bacillus, Coprothermobacter, Rhodobacter, Pseudomonas, Achromobacter, Desulfitobacter, Desulfosporosinus, T78, Methanobacterium, Methanosaeta, etc). Together with nutrients, bioaugmentation with biosurfactant producing and hydrocarbon utilizing indigenous Bacillus strains resulted in 57-75% TPH reduction. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis revealed enhanced gene allocation for transporters (0.45-3.07%), ABC transporters (0.38-2.07%), methane (0.16-1.06%), fatty acid (0.018-0.15%), nitrogen (0.07-0.17%), butanoate (0.06-0.35%), propanoate (0.004-0.26%) metabolism and some xenobiotics (0.007-0.13%) degradation. This study indicated that nutrient-induced community dynamics of native microorganisms and their metabolic interplay within oil refinery sludge could be a driving force behind accelerated bioremediation.
通过生物刺激和生物增强方法，研究了开发用于处理富含碳氢化合物的石油精炼厂废物的工程生物修复策略的可能性。通过在污泥中添加磷酸盐、硝酸盐或硝酸盐+磷酸盐，可实现总石油烃（TPH）衰减增强（46-55%），并增加（高达 12%）发酵、烃降解、硫酸盐还原、CO 同化和产甲烷微生物（芽孢杆菌、Coprothermobacter、Rhodobacter、Pseudomonas、Achromobacter、Desulfitobacter、Desulfosporosinus、T78、Methanobacterium、Methanosaeta 等）的丰度。与营养物一起，通过生物增强用生物表面活性剂产生和利用烃的本土芽孢杆菌菌株可实现 57-75%的 TPH 减少。未观察到状态重建的群落系统发育研究（PICRUSt）分析表明，转运蛋白（0.45-3.07%）、ABC 转运蛋白（0.38-2.07%）、甲烷（0.16-1.06%）、脂肪酸（0.018-0.15%）、氮（0.07-0.17%）、丁酸盐（0.06-0.35%）、丙酸盐（0.004-0.26%）代谢和一些外来化合物（0.007-0.13%）降解的基因分配增强。本研究表明，营养物诱导的土著微生物群落动态及其在炼油厂污泥中的代谢相互作用可能是加速生物修复的驱动力。
