Chen Wenhua, Wang Jingxuan, Song Jia, Sun Qingmei, Zhu Bo, Qin Luping
School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, P. R. China.
ACS Omega. 2023 Mar 24;8(13):12489-12500. doi: 10.1021/acsomega.3c00608. eCollection 2023 Apr 4.
Both the biosynthesis and array of bioactive and medicinal compounds in plants can be influenced by interactions with endophytic and exogenous fungi. However, the composition of endophytic and exogenous fungal communities associated with many medicinal plants is unknown, and the mechanism by which these fungi stimulate the secondary metabolism of host plants is unclear. In this study, we conducted a correlative analysis between endophytic and exogenous fungi and dendrobine and biomass accumulation in across five Chinese habitats: wild Danxia rock, greenhouse-associated large Danxia stone, broken Danxia stone, broken coarse sandstone, and wood spile. Across habitats, fungal communities exhibited significant differences. The abundances of , , and were higher in wild habitats than in greenhouse habitats. Wild habitats were host to a higher diversity and richness of exogenous fungi than were greenhouse habitats. However, there was no significant difference in endophytic fungal diversity between habitats. The differences between the fungal communities' effects on the dendrobine content and biomass of were attributable to the composition of endophytic and exogenous fungi. Exogenous fungi had a greater impact than endophytic fungi on the accumulation of fresh weight (FW) and dendrobine in . Furthermore, samples with higher exogenous fungal richness and diversity exhibited higher dendrobine content and FW. was the only genus to be significantly positively correlated with both FW and dendrobine content. A total of 86 strains of endophytic fungi were isolated from the roots, stems, and leaves of , of which 8 strains were found to be symbiotic with tissue-cultured seedlings. The strain DN14 () was found to promote not only biomass accumulation (11.44%) but also dendrobine content (33.80%) in tissue-cultured seedlings. The results of this study will aid in the development of strategies to increase the production of dendrobine in . This work could also facilitate the screening of beneficial endophytic and exogenous fungal probiotics for use as biofertilizers in .
植物中生物活性和药用化合物的生物合成及排列都可能受到与内生真菌和外源真菌相互作用的影响。然而，许多药用植物相关的内生真菌和外源真菌群落的组成尚不清楚，且这些真菌刺激宿主植物次生代谢的机制也不明确。在本研究中，我们对中国五个栖息地（野生丹霞岩、温室相关的大丹霞石、破碎的丹霞石、破碎的粗砂岩和木桩）的内生真菌和外源真菌与石蒜碱及生物量积累之间进行了相关性分析。在不同栖息地中，真菌群落表现出显著差异。野生栖息地中，[具体真菌种类1]、[具体真菌种类2]和[具体真菌种类3]的丰度高于温室栖息地。与温室栖息地相比，野生栖息地中外源真菌的多样性和丰富度更高。然而，不同栖息地之间内生真菌多样性没有显著差异。真菌群落对石蒜碱含量和[植物名称]生物量的影响差异归因于内生真菌和外源真菌的组成。外源真菌对[植物名称]鲜重（FW）和石蒜碱积累的影响大于内生真菌。此外，外源真菌丰富度和多样性较高的[植物名称]样本表现出更高的石蒜碱含量和FW。[具体真菌属名]是唯一与FW和石蒜碱含量均显著正相关的属。从[植物名称]的根、茎和叶中总共分离出86株内生真菌菌株，其中8株被发现与[植物名称]组培苗共生。菌株DN14（[具体菌种名]）被发现不仅能促进[植物名称]组培苗的生物量积累（11.44%），还能提高石蒜碱含量（33.80%）。本研究结果将有助于制定提高[植物名称]中石蒜碱产量的策略。这项工作还可以促进筛选有益的内生真菌和外源真菌益生菌，用于[植物名称]的生物肥料。
