Division of Rheumatology, Tufts Medical Center, Boston, MA, USA.
J Sport Rehabil. 2010 Nov;19(4):411-21. doi: 10.1123/jsr.19.4.411.
Joint trauma is a risk factor for osteoarthritis (OA), which is becoming an increasingly important orthopedic concern for athletes and nonathletes alike. For advances in OA prevention, diagnosis, and treatment to occur, a greater understanding of the biochemical environment of the affected joint is needed.
To demonstrate the potential of a biochemical technique to enhance our understanding of and diagnostic capabilities for osteoarthritis.
Cross-sectional.
Outpatient orthopedic practice.
8 subjects: 4 OA-knee participants (65 ± 6 y of age) and 4 normal-knee participants (54 ± 10 y) with no history of knee OA based on bilateral standing radiographs.
The independent variable was group (OA knee, normal knee).
16 knee synovial-protein concentrations categorized as follows: 4 as pro-inflammatory, or catabolic, cytokines; 5 as anti-inflammatory, or protective, cytokines; 3 as catabolic enzymes; 2 as tissue inhibitors of metalloproteinases [TIMPs]; and 2 as adipokines.
Two anti-inflammatory cytokines (interleukin [IL]-13 and osteoprotegerin) and a pro-inflammatory cytokine (IL-1β) were significantly lower in the OA knees. Two catabolic enzymes (matrix metalloproteinase [MMP]-2 and MMP-3) were significantly elevated in OA knees. TIMP-2, an inhibitor of MMPs, was significantly elevated in OA knees.
Six of the 16 synovial-fluid proteins were significantly different between OA knees and normal knees in this study. Future research using a similar multiplex ELISA approach or other proteomic techniques may enable researchers and clinicians to develop more accurate biochemical profiles of synovial fluid to help diagnose OA, identify subsets of OA or individual characteristics, guide clinical decisions, and identify patients at risk for OA after knee injury.
关节损伤是骨关节炎（OA）的一个风险因素，OA 正成为运动员和非运动员都越来越关注的重要骨科疾病。为了推进 OA 的预防、诊断和治疗进展，我们需要更深入地了解患病关节的生化环境。
展示一种生化技术在增强我们对 OA 的理解和诊断能力方面的潜力。
横断面研究。
门诊骨科诊所。
8 名受试者：4 名膝关节 OA 参与者（65 ± 6 岁）和 4 名膝关节正常参与者（54 ± 10 岁），根据双侧站立位 X 线片，他们均无膝关节 OA 病史。
自变量为组别（OA 膝关节、正常膝关节）。
16 种膝关节滑膜蛋白浓度，分为以下 4 类：4 种促炎或分解代谢细胞因子；5 种抗炎或保护细胞因子；3 种分解代谢酶；2 种金属蛋白酶组织抑制剂[TIMP]；2 种脂肪因子。
OA 膝关节中的两种抗炎细胞因子（白细胞介素[IL]-13 和护骨素）和一种促炎细胞因子（IL-1β）明显降低。两种分解代谢酶（基质金属蛋白酶[MMP]-2 和 MMP-3）在 OA 膝关节中明显升高。MMP 抑制剂 TIMP-2 在 OA 膝关节中明显升高。
在这项研究中，OA 膝关节和正常膝关节的 16 种滑液蛋白中有 6 种存在显著差异。未来使用类似的多重 ELISA 方法或其他蛋白质组学技术的研究可能使研究人员和临床医生能够开发出更准确的滑液生化特征谱，以帮助诊断 OA、识别 OA 的亚组或个体特征、指导临床决策，并识别膝关节损伤后发生 OA 的风险患者。
