Associated Tissue Bank, Faculty of Medicine, P. J. Safarik University, L. Pasteur University Hospital, Kosice, Slovakia.
Department of Orthopaedics and Traumatology, Faculty of Medicine, P. J. Safarik University, L. Pasteur University Hospital, Kosice, Slovakia.
Cartilage. 2021 Dec;13(2_suppl):1421S-1430S. doi: 10.1177/1947603520946385. Epub 2020 Aug 4.
Osteoarthritis (OA) commonly affects weight-bearing joints and is characterized by articular cartilage breakdown combined with osteophyte formation at the joint margins and chronic nonspecific inflammation of synovium. Understanding the profile of inflammation in a patient population is an essential starting point to predict or prevent OA progression. The aim of this study was to identify the profile of selected biomolecules in synovial fluid (SF) and investigate the correlation according to gender, age, and severity of the disease within patients from among the general knee OA population.
In our study SF samples were aspirated from the knees of 65 OA patients (46 patients with early knee OA and 19 patients with end-stage knee OA according to the Kellgren-Lawrence grading scale). The concentration of interleukins (IL-6, IL-8), matrix metalloproteinases (MMP-1, MMP-3, MMP-13), MMPs inhibitors (TIMP-1, TIMP-2), cartilage oligomeric matrix protein (COMP), and adiponectin was analyzed using a multiplex ELISA-based approach.
Our results indicate significant linear correlation of MMP-13 and COMP concentration with age ( < 0.05), but not with OA severity. In fact, 3 of the examined biomolecules, MMP-3 ( < 0.01), TIMP-1 ( < 0.01), and COMP ( < 0.05) significantly correlate with the grade of knee OA and might be associated with OA severity.
骨关节炎（OA）通常影响负重关节，其特征是关节软骨破坏，同时伴有关节边缘骨赘形成和滑膜慢性非特异性炎症。了解患者人群中的炎症特征是预测或预防 OA 进展的重要起点。本研究旨在确定滑液（SF）中选定生物分子的特征，并根据性别、年龄以及患者关节 OA 的严重程度，分析这些特征之间的相关性。
在本研究中，我们从 65 例 OA 患者（46 例早期膝 OA 患者和 19 例晚期膝 OA 患者，根据 Kellgren-Lawrence 分级标准）的膝关节中抽取 SF 样本。采用基于多重 ELISA 的方法分析白细胞介素（IL-6、IL-8）、基质金属蛋白酶（MMP-1、MMP-3、MMP-13）、MMP 抑制剂（TIMP-1、TIMP-2）、软骨寡聚基质蛋白（COMP）和脂联素的浓度。
我们的结果表明，MMP-13 和 COMP 浓度与年龄呈显著线性相关（<0.05），但与 OA 严重程度无关。实际上，在 3 种检查的生物分子中，MMP-3（<0.01）、TIMP-1（<0.01）和 COMP（<0.05）与膝 OA 分级显著相关，可能与 OA 严重程度相关。
