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腰果苹果渣作为马克斯克鲁维酵母 CE025 生产乙醇的糖源。

Cashew apple bagasse as a source of sugars for ethanol production by Kluyveromyces marxianus CE025.

机构信息

Departamento de Agrotecnologia e Ciências Sociais, Universidade Federal Rural do Semi-Árido, Av. Francisco Mota, 572, Costa e Silva, Mossoró, RN, 59.625-900, Brazil.

出版信息

J Ind Microbiol Biotechnol. 2011 Aug;38(8):1099-107. doi: 10.1007/s10295-010-0889-0. Epub 2010 Nov 30.

DOI:10.1007/s10295-010-0889-0
PMID:21116682
Abstract

The potential of cashew apple bagasse as a source of sugars for ethanol production by Kluyveromyces marxianus CE025 was evaluated in this work. This strain was preliminarily cultivated in a synthetic medium containing glucose and xylose and was able to produce ethanol and xylitol at pH 4.5. Next, cashew apple bagasse hydrolysate (CABH) was prepared by a diluted sulfuric acid pretreatment and used as fermentation media. This hydrolysate is rich in glucose, xylose, and arabinose and contains traces of formic acid and acetic acid. In batch fermentations of CABH at pH 4.5, the strain produced only ethanol. The effects of temperature on the kinetic parameters of ethanol fermentation by K. marxianus CE025 using CABH were also evaluated. Maximum specific growth rate (μ(max)), overall yields of ethanol based on glucose consumption [Formula: see text] and based on glucose + xylose consumption (Y ( P/S )), overall yield of ethanol based on biomass (Y ( P/X )), and ethanol productivity (P (E)) were determined as a function of temperature. Best results of ethanol production were achieved at 30°C, which is also quite close to the optimum temperature for the formation of biomass. The process yielded 12.36 ± 0.06 g l(-1) of ethanol with a volumetric production rate of 0.257 ± 0.002 g l(-1) h(-1) and an ethanol yield of 0.417 ± 0.003 g g(-1) glucose.

摘要

本工作评估了腰果苹果渣作为马克斯克鲁维酵母 CE025 生产乙醇的糖源的潜力。该菌株在含有葡萄糖和木糖的合成培养基中进行了初步培养,能够在 pH4.5 下生产乙醇和木糖醇。然后,通过稀硫酸预处理制备了腰果苹果渣水解物(CABH),并将其用作发酵培养基。该水解物富含葡萄糖、木糖和阿拉伯糖,并且含有痕量的甲酸和乙酸。在 pH4.5 下的 CABH 分批发酵中,该菌株仅产生乙醇。还评估了温度对 K. marxianus CE025 利用 CABH 发酵乙醇的动力学参数的影响。最大比生长速率(μ(max))、基于葡萄糖消耗的乙醇总体产率[公式:见文本]和基于葡萄糖+木糖消耗的乙醇总体产率(Y(P/S))、基于生物质的乙醇总体产率(Y(P/X))和乙醇生产率(P(E))均作为温度的函数进行了测定。在 30°C 下获得了最佳的乙醇生产结果,这也非常接近生物量形成的最佳温度。该工艺产生了 12.36±0.06g l(-1)的乙醇,体积产率为 0.257±0.002g l(-1) h(-1),乙醇得率为 0.417±0.003g g(-1)葡萄糖。

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