Research Institute for Biological Sciences, Tokyo University of Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan.
Vet Res Commun. 2011 Jan;35(1):47-54. doi: 10.1007/s11259-010-9454-6. Epub 2010 Dec 1.
High-mobility group box 1 (HMGB1) protein is the major component of the nonhistone nuclear protein group and is involved in nucleosome stabilization and transcription regulation. HMGB1 has recently been focused on as a proinflammatory cytokine associated with various inflammatory diseases and as a target of anti-inflammatory therapy. Mastitis, a serious inflammatory disease of dairy cows, is caused by infection of the mammary gland and has detrimental effects on the quantity and quality of milk. By detecting the presence of HMGB1 in milk, we investigated the correlation between HMGB1 concentration and the severity of bovine mastitis, which was determined using the California Mastitis Test and somatic cell count (SCC). We detected a substantial amount of HMGB1 in mastitic milk but not in the milk from normal cows. We used the Spearman rank correlation coefficient to assess the relationship between HMGB1 concentration and SCC and found a significant correlation (n = 12, r = 0.975). Thus, we confirmed the positive correlation between HMGB1 concentration and SCC in milk, i.e., the severity of mastitis, which suggested that HMGB1 in milk is a new indicator of bovine mastitis.
高迁移率族蛋白 B1（HMGB1）是核蛋白组中非组蛋白的主要成分，参与核小体稳定和转录调控。HMGB1 最近被认为是一种与各种炎症性疾病相关的促炎细胞因子，也是抗炎治疗的靶点。乳腺炎是奶牛的一种严重炎症性疾病，由乳腺感染引起，对牛奶的数量和质量都有不利影响。通过检测牛奶中 HMGB1 的存在，我们研究了 HMGB1 浓度与奶牛乳腺炎严重程度之间的相关性，乳腺炎严重程度是通过加利福尼亚乳腺炎检测和体细胞计数（SCC）来确定的。我们在乳腺炎牛奶中检测到大量的 HMGB1，但在正常牛奶中没有检测到。我们使用斯皮尔曼等级相关系数来评估 HMGB1 浓度与 SCC 之间的关系，发现两者之间存在显著相关性（n = 12，r = 0.975）。因此，我们证实了牛奶中 HMGB1 浓度与 SCC 之间的正相关关系，即乳腺炎的严重程度，这表明牛奶中的 HMGB1 是一种新的奶牛乳腺炎指标。
