Section of Molecular Cytology and Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
J Microsc. 2011 Feb;241(2):111-8. doi: 10.1111/j.1365-2818.2010.03437.x. Epub 2010 Sep 27.
The phenomenon of resonance energy transfer first described by Theodor Förster presents the opportunity of retrieving information on molecular proximity, orientation and conformation on the nanometre scale from (living) samples with conventional fluorescence microscopes (or even macroscopic devices). During the past 10 years Förster (or fluorescence) resonance energy transfer (FRET) microscopy has been revolutionized by the vast progress in fluorescent protein and in situ fluorescent labelling technology as well as by the commercial availability of advanced quantitative microscopy instrumentation. FRET microscopy is now routinely used in modern cell biology research. This short review will guide the reader through the most established FRET microscopy techniques, their inherent strengths and limitations, potential pitfalls, and assist the reader in making an educated choice on the FRET microscopy method most suited for their specific application.
福斯特（Förster）首次描述的共振能量转移现象为利用传统荧光显微镜（甚至宏观设备）从（活）样本中获取纳米级分子接近度、取向和构象信息提供了机会。在过去的 10 年中，荧光蛋白和原位荧光标记技术的巨大进步以及先进定量显微镜仪器的商业化，使福斯特（Förster 或荧光）共振能量转移（FRET）显微镜发生了革命性变化。FRET 显微镜现在已在现代细胞生物学研究中得到常规应用。这篇简短的综述将引导读者了解最成熟的 FRET 显微镜技术、它们的固有优势和局限性、潜在的陷阱，并帮助读者在最适合其特定应用的 FRET 显微镜方法中做出明智的选择。
