Osano E, Tanaka T, Ozeki M, Makita I, Moriyama T
Department of Microbiology, School of Dentistry, Aichi-Gakuin University.
Microbiol Immunol. 1990;34(2):211-9.
Employing twenty fresh oral isolates of Streptococcus intermedius, studies were carried out to characterize serological relations among the isolates and also between the isolates and the strains of bacterial species closely related to S. intermedius. The Rantz-Randall extracts from the cells were used as antigens. The anti-rabbit serum raised against S. intermedius ATCC 27335T reacted with the cell extracts from only three strains of the isolates, which were designated serogroup I strains. The other isolates were classified into four serogroups, I, III, IV, and V, which specifically reacted with the cell extracts from the homologous serogroup strains. However, the serogroup II antiserum formed in immunodiffusion a common precipitin line between the extracts from the cells of serogroups II and I. The serogroups I, III, IV, and V antisera reacted with none of the extracts from the bacterial cells closely related to S. intermedius, which included Streptococcus anginosus ATCC 33397T, Streptococcus constellatus ATCC 27823T, three NCTC strains of "Streptococcus milleri," and three ATCC strains of Streptococcus MG. The precipitin line formed by the homologous reaction of the serogroup II antiserum was found to be a reaction of identity with that formed by the extract from "S. milleri" NCTC 10708. Conversely, the antiserum against NCTC 10708 strain did not react with the cell extracts of serogroup II.
利用20株中间型链球菌的新鲜口腔分离株，开展了相关研究，以表征这些分离株之间以及这些分离株与和中间型链球菌密切相关的细菌物种菌株之间的血清学关系。细胞的兰茨-兰德尔提取物用作抗原。针对中间型链球菌ATCC 27335T产生的抗兔血清仅与三株分离株的细胞提取物发生反应，这三株分离株被指定为血清群I菌株。其他分离株被分为四个血清群，即II、III、IV和V，它们分别与同源血清群菌株的细胞提取物发生特异性反应。然而，血清群II抗血清在免疫扩散中在血清群II和I的细胞提取物之间形成了一条共同沉淀线。血清群I、III、IV和V抗血清与和中间型链球菌密切相关的细菌细胞提取物均无反应，这些细菌包括咽峡炎链球菌ATCC 33397T、星座链球菌ATCC 27823T、三株“米勒链球菌”的NCTC菌株以及三株链球菌MG的ATCC菌株。发现血清群II抗血清同源反应形成的沉淀线与“米勒链球菌”NCTC 10708提取物形成的沉淀线呈同一反应。相反，针对NCTC 10708菌株的抗血清与血清群II的细胞提取物无反应。
