Fujii Y
Aichi Gakuin Daigaku Shigakkai Shi. 1989 Mar;27(1):137-51.
Human oral isolates of Streptococcus intermedius, which were biochemically identified, have been classified into 5-Serogroups (I-V). Selecting some representive stock cultures together with the type strain ATCC 27335, comparisons were carried out between them in terms of cell extractable proteins, G + C mol% values, DNA-DNA homology and DNA restriction fragments. Since the taxon of S. intermedius has not been clarified, comparison was also made between the above strains and the type strains of the "Streptococcus anginosus-milleri Group", Streptococcus anginosus, "Streptococcus milleri" and Streptococcus constellatus, which were thought to be related to S. intermedius. 1. G + C mol% of S. intermedius ATCC 27335 was 38.0 +/- 0.28. The values of all the isolates tested ranged from 37.0 to 38.8. On the other hand, the values of the type strains of the "S. anginosus-milleri Group" were 38.0-38.8. 2. Under stringent conditions of DNA-DNA hybridization, all the isolates showed more than 66.7% homology with S. intermedius ATCC 27335, demonstrating that these strains belonged to the same species. On the other hand, S. anginosus ATCC 33397, "S. milleri" NCTC 11169 and S. constellatus ATCC 27823 hybridized at levels of 50%, 53% and 28%, respectively. Consequently, it was impossible to classify these strains as being the same species as S. intermedius. 3. When the DNA restriction fragments were compared by PAGE, the strains of Serogroups I and V showed the same pattern, respectively. The strains of Serogroups II and III, however, showed two different patterns, and these of Serogroup IV showed five different patterns. DNA fragments specific to S. intermedius could not be identified. 4. When cell extractable groups were compared by SDS-PAGE, the strains of Serogroups I and V showed identical patterns. The strains of Serogroup III showed 2 different patterns and those of Serogroup IV showed 5 patterns. These results reflected those of the DNA restriction analysis. However, the strains of Serogroup II showed the same protein pattern, although 2 different patterns were observed in DNA restriction fragments. Unique proteins, which were not detected in the proteins extracted from the type strains of the "S. anginosus-milleri Group", were observed in the cell proteins of S. intermedius strains. 5. The results of DNA-DNA hybridization and the existence of unique extractable proteins together with the chondroitinase productivity suggest that the strains of S. intermedius might represent a particular taxon among the strains of the "S. anginosus-milleri Group".(ABSTRACT TRUNCATED AT 400 WORDS)
经生化鉴定的人源口腔中间型链球菌分离株已被分为5个血清群（I - V）。选取一些具有代表性的菌株以及模式菌株ATCC 27335，对它们在细胞可提取蛋白、G + C摩尔百分比值、DNA - DNA同源性和DNA限制性片段方面进行了比较。由于中间型链球菌的分类尚未明确，还对上述菌株与“咽峡炎链球菌 - 米勒链球菌群”、咽峡炎链球菌、“米勒链球菌”和星座链球菌的模式菌株进行了比较，这些菌株被认为与中间型链球菌有关。1. 中间型链球菌ATCC 27335的G + C摩尔百分比为38.0±0.28。所有测试分离株的值在37.0至38.8之间。另一方面，“咽峡炎链球菌 - 米勒链球菌群”模式菌株的值为38.0 - 38.8。2. 在严格的DNA - DNA杂交条件下，所有分离株与中间型链球菌ATCC 27335的同源性均超过66.7%，表明这些菌株属于同一物种。另一方面，咽峡炎链球菌ATCC 33397、“米勒链球菌”NCTC 11169和星座链球菌ATCC 27823的杂交水平分别为50%、53%和28%。因此，无法将这些菌株归类为与中间型链球菌同一物种。3. 通过聚丙烯酰胺凝胶电泳（PAGE）比较DNA限制性片段时，血清群I和V的菌株分别显示出相同的模式。然而，血清群II和III的菌株显示出两种不同的模式，血清群IV的菌株显示出五种不同的模式。未鉴定出中间型链球菌特有的DNA片段。4. 通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）比较细胞可提取组分时，血清群I和V的菌株显示出相同的模式。血清群III的菌株显示出2种不同的模式，血清群IV的菌株显示出5种模式。这些结果反映了DNA限制性分析的结果。然而，血清群II的菌株显示出相同的蛋白质模式，尽管在DNA限制性片段中观察到2种不同的模式。在中间型链球菌菌株的细胞蛋白中观察到了在“咽峡炎链球菌 - 米勒链球菌群”模式菌株提取的蛋白质中未检测到的独特蛋白质。5. DNA - DNA杂交结果、独特可提取蛋白质的存在以及软骨素酶的产生表明，中间型链球菌菌株可能代表“咽峡炎链球菌 - 米勒链球菌群”菌株中的一个特定分类单元。（摘要截选至400字）
