Tsuboi K, Yamaoka S, Maki M, Ohshio G, Tobe T, Hatanaka M
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Biochem Biophys Res Commun. 1990 May 16;168(3):1163-70. doi: 10.1016/0006-291x(90)91151-h.
The wound healing process is initiated as soon as tissue is injured. Herein, we demonstrate that c-fos and c-myc mRNA transcripts are promptly increased in the wounded tissue in vivo and in vitro. A buffer solution from scraped serum-starved quiescent fibroblasts, when added to resting fibroblasts, caused the increase of c-fos and c-myc mRNA among the indicator cells. Soluble factors contained in the wounding supernatant are responsible for these phenomena and we call them wounding factors. Addition of proteinase inhibitors to the culture medium drastically reduced the c-fos mRNA induction by the wounding factors. Exogenously added trypsin or thrombin mimicked the activity of wounding factors. These results suggest that wounding causes soluble factors including various proteinases to be released from the damaged cells, which trigger the adjacent cells to respond to the injury.
一旦组织受到损伤，伤口愈合过程就会启动。在此，我们证明在体内和体外受伤组织中，c-fos和c-myc信使核糖核酸转录本会迅速增加。来自刮取的血清饥饿静止成纤维细胞的缓冲溶液，当添加到静止成纤维细胞中时，会导致指示细胞中c-fos和c-myc信使核糖核酸增加。伤口上清液中含有的可溶性因子导致了这些现象，我们将其称为伤口因子。向培养基中添加蛋白酶抑制剂可显著降低伤口因子对c-fos信使核糖核酸的诱导作用。外源添加的胰蛋白酶或凝血酶模拟了伤口因子的活性。这些结果表明，伤口会导致包括各种蛋白酶在内的可溶性因子从受损细胞中释放出来，从而触发相邻细胞对损伤做出反应。
