Departamento de Microbiologia Médica, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2010 Nov;105(7):931-4. doi: 10.1590/s0074-02762010000700017.
A total of 138 isolates, 118 methicillin-resistant Staphylococcus aureus (MRSA) isolates (staphylococcal cassette chromosome type II, 20 isolates, type III, 39 isolates and type IV, 59 isolates) and 20 methicillin-sensitive S. aureus isolates were evaluated by phenotypic methods: cefoxitin and oxacillin disk diffusion (DD), agar dilution (AD), latex agglutination (LA), oxacillin agar screening (OAS) and chromogenic agar detection. All methods showed 100% specificity, but only the DD tests presented 100% sensitivity. The sensitivity of the other tests ranged from 82.2% (OAS)-98.3% (AD). The LA test showed the second lowest sensitivity (86.4%). The DD test showed high accuracy in the detection of MRSA isolates, but there was low precision in the detection of type IV isolates by the other tests, indicating that the genotypic characteristics of the isolates should be considered.
共评估了 138 株分离株，包括 118 株耐甲氧西林金黄色葡萄球菌（MRSA）分离株（葡萄球菌盒式染色体 II 型 20 株、III 型 39 株和 IV 型 59 株）和 20 株甲氧西林敏感的 S. aureus 分离株。采用表型方法进行评估：头孢西丁和苯唑西林纸片扩散（DD）、琼脂稀释（AD）、乳胶凝集（LA）、苯唑西林琼脂筛选（OAS）和显色琼脂检测。所有方法的特异性均为 100%，但只有 DD 试验的敏感性为 100%。其他试验的敏感性范围为 82.2%（OAS）-98.3%（AD）。LA 试验的敏感性最低（86.4%）。DD 试验在检测 MRSA 分离株方面具有较高的准确性，但其他试验在检测 IV 型分离株时精度较低，表明应考虑分离株的基因型特征。
