Nyquist Battie C, Moran N
School of Basic Life Sciences, University of Missouri-Kansas City 64108.
Cardiovasc Res. 1990 Apr;24(4):335-9. doi: 10.1093/cvr/24.4.335.
The aim of the study was to determine the significance of adrenergic nerve associated acetylcholinesterase for the pool of total acetylcholinesterase molecules.
Acetylcholinesterase was analysed after destruction of adrenergic nerves by 6-hydroxydopamine or bilateral stellate sympathectomy. Effectiveness of treatment was verified by determining noradrenaline concentrations in right ventricle. Acetylcholinesterase activity was assayed in homogenates of atria and portions of left ventricular free wall.
Adult male Sprague-Dawley rats were used, weight 225-260 g, n = 5 per experimental group.
Sympathectomy caused a small decrease in acetylcholinesterase activity, due to a decrease in the activity of the tetrameric globular form of the enzyme. Choline acetylcholinesterase activity was not altered by sympathectomy, which is an indication that cholinergic nerves were not affected.
The contribution of adrenergic neurones to the cardiac pool of acetylcholinesterase is measurable and consists primarily of the tetrameric globular form of the enzyme.
本研究旨在确定肾上腺素能神经相关乙酰胆碱酯酶对总乙酰胆碱酯酶分子池的意义。
在用6-羟基多巴胺破坏肾上腺素能神经或双侧星状交感神经切除术后，对乙酰胆碱酯酶进行分析。通过测定右心室中的去甲肾上腺素浓度来验证治疗效果。在心房和左心室游离壁部分的匀浆中测定乙酰胆碱酯酶活性。
使用成年雄性Sprague-Dawley大鼠，体重225 - 260克，每组实验n = 5只。
交感神经切除术导致乙酰胆碱酯酶活性略有下降，这是由于该酶的四聚体球状形式的活性降低所致。胆碱乙酰胆碱酯酶活性未因交感神经切除术而改变，这表明胆碱能神经未受影响。
肾上腺素能神经元对心脏乙酰胆碱酯酶池的贡献是可测量的，并且主要由该酶的四聚体球状形式组成。
