Pantou A L, Markopoulou C E, Dereka X E, Vavouraki H, Mamalis A, Vrotsos I A
Department of Periodontology, School of Dentistry, University of Athens, Athens, Greece.
Cell Tissue Bank. 2012 Mar;13(1):81-8. doi: 10.1007/s10561-010-9231-3. Epub 2010 Dec 1.
The prominent purpose of the study was the evaluation of the in vitro mitogenic effect of three different homologous platelet-rich plasma (PRP) preparations (PRPa, PRPb, PRPc) on three different lines of periodontal ligament (PDL) cells (PDL(1,2,3)), cultured alone or in combination with a demineralized freeze-dried allograft (DFBA). PDL cell cultures were derived from the mid root of three maxillary caries-free premolars extracted for orthodontic reasons. Cells were grown and reached confluence. To evaluate the mitogenic effect of all exogenous factors (PRPa, PRPb, PRPc and DFBA) on PDL cells, specific number of cells (10.000/well) was cultured in the presence or absence of the above factors. Each PRP preparation (5% v/v) was added in all cell lines, in the absence or presence of 10 mg/ml of DFBA. The cells were also treated with 25 ng/ml bFGF (positive control). The mitogenic effect was evaluated 24 h after incubation, using the Trypan blue exclusion assay. The results revealed that all PRP preparations act as potent mitogens as they significantly induced cell proliferation on PDL(1,2,3) lines. All PRP preparations when added alone in the PDL cell cultures, exhibited a significant advantage over the positive control (bFGF). The addition of DFBA to PRP did not influence significantly cell proliferation in all cell lines, comparatively to PRP alone, at the time -period studied. The findings of this study demonstrate the beneficial role of PRP alone or combined with the bone graft on periodontal ligament cells in vitro, suggesting that it may be considered as a potential biological approach in periodontal regeneration.
该研究的主要目的是评估三种不同的同源富血小板血浆（PRP）制剂（PRPa、PRPb、PRPc）对三种不同牙周膜（PDL）细胞系（PDL(1,2,3)）的体外促有丝分裂作用，这些细胞单独培养或与脱矿冻干同种异体骨（DFBA）联合培养。PDL细胞培养物来源于因正畸原因拔除的三颗上颌无龋前磨牙的牙根中部。细胞生长至汇合。为了评估所有外源性因素（PRPa、PRPb、PRPc和DFBA）对PDL细胞的促有丝分裂作用，在有或无上述因素的情况下培养特定数量的细胞（10000个/孔）。在所有细胞系中添加每种PRP制剂（5% v/v），有无10 mg/ml的DFBA。细胞还用25 ng/ml碱性成纤维细胞生长因子（bFGF，阳性对照）处理。孵育24小时后，使用台盼蓝排斥试验评估促有丝分裂作用。结果显示，所有PRP制剂均作为强效促有丝分裂原，因为它们显著诱导PDL(1,2,3)细胞系的细胞增殖。所有PRP制剂单独添加到PDL细胞培养物中时，相对于阳性对照（bFGF）表现出显著优势。在研究的时间段内，与单独使用PRP相比，向PRP中添加DFBA对所有细胞系的细胞增殖没有显著影响。本研究结果证明了PRP单独或与骨移植联合对体外牙周膜细胞的有益作用，表明它可被视为牙周再生的一种潜在生物学方法。
