Markopoulou C E, Dereka X E, Vavouraki H N, Pepelassi E E, Mamalis A A, Karoussis I K, Vrotsos I A
Department of Periodontics, School of Dentistry, University of Athens, 2 Thivon Street, Goudi, 11527, Athens, Greece.
Growth Factors. 2011 Feb;29(1):14-20. doi: 10.3109/08977194.2010.533663. Epub 2010 Dec 3.
Various techniques and materials have been proposed for the treatment of periodontal defects. In periodontal regeneration, periodontal ligament (PDL) cell differentiation as well as certain growth factors and their delivery system applied are critical. The purpose of this study was to evaluate the in vitro effect of recombinant human transforming growth factor-beta 1 (rhTGF-β1) combined with two different bone grafts on human PDL (hPDL) cell differentiation. The hPDL cells were treated with TGF-β1 alone or in combination with a calcified freeze-dried bone allograft (FDBA) and a porous biphasic calcium phosphate (BC) bone graft. Cell differentiation effect was estimated by measuring alkaline phosphatase (ALPase) activity and osteocalcin secretion. Results demonstrated that rhTGF-β1 alone or in combination with FDBA and BC provoked a significant (p<0.05) increase in ALPase activity as compared with controls. The findings of this study confirmed the beneficial role of rhTGF-β1 combined with FDBA and BC as carriers in periodontal regeneration.
已经提出了多种用于治疗牙周缺损的技术和材料。在牙周再生中，牙周膜（PDL）细胞分化以及应用的某些生长因子及其递送系统至关重要。本研究的目的是评估重组人转化生长因子-β1（rhTGF-β1）与两种不同骨移植材料联合应用对人牙周膜（hPDL）细胞分化的体外作用。hPDL细胞分别用单独的TGF-β1或与钙化冻干同种异体骨（FDBA）和多孔双相磷酸钙（BC）骨移植材料联合处理。通过测量碱性磷酸酶（ALPase）活性和骨钙素分泌评估细胞分化效果。结果表明，与对照组相比，单独使用rhTGF-β1或与FDBA和BC联合使用均能显著（p<0.05）提高ALPase活性。本研究结果证实了rhTGF-β1与FDBA和BC联合作为载体在牙周再生中的有益作用。
