Clendenon N R, Allen N, Gordon W A, Bingham W G
J Neurosurg. 1978 Oct;49(4):563-8. doi: 10.3171/jns.1978.49.4.0563.
The specific activity of the membrane-bound enzyme, Na+-K+-activated adenosine triphosphatase (ATPase), has been shown to be decreased following experimental impact injury (400 gm-cm) to the spinal cord in dogs. The prompt and significant (p less than 0.01) fall in activity was evident as early as 5 minutes after injury, and remained at 56% to 67% of control for the 1-hour period studied. This decrease was most prominent in the central core of the traumatized segments of spinal cord. Central core samples, excised immediately adjacent to the trauma site, gave values for the Na+-K+-activated enzyme intermediate to those of the trauma and control sites. For these same samples, the activity of the Mg+2-dependent ATPase did not change appreciably. No alterations were observed in the tissue surrounding the zone of maximum injury at these early time periods. The relationship of membrane-bound enzyme alterations to blood flow, clotting mechanisms, and abnormal free radical reactions are briefly discussed.
实验性撞击伤（400克 - 厘米）犬脊髓后，膜结合酶钠钾激活三磷酸腺苷酶（ATP酶）的比活性已显示降低。早在损伤后5分钟，酶活性就迅速且显著（p小于0.01）下降，在研究的1小时内，其活性维持在对照值的56%至67%。这种下降在脊髓创伤节段的中央核心最为明显。紧邻创伤部位立即切除的中央核心样本，其钠钾激活酶的值介于创伤部位和对照部位之间。对于这些相同样本，镁离子依赖性ATP酶的活性没有明显变化。在这些早期时间段，最大损伤区域周围的组织未观察到改变。本文简要讨论了膜结合酶改变与血流、凝血机制及异常自由基反应的关系。
