State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, China.
Colloids Surf B Biointerfaces. 2011 Jun 15;85(1):32-9. doi: 10.1016/j.colsurfb.2010.10.042. Epub 2010 Nov 3.
Hydrophilicity improvement and bioactive surface design of poly(ɛ-caprolactone) (PCL) grafts are of key importance for their application in tissue engineering. Herein, we develop a convenient approach for achieving stable hydrophilic surfaces by modifying electrospun PCL grafts with a class II hydrophobin (HFBI) coating. Static water contact angles (WCA) demonstrated the conversion of the PCL grafts from hydrophobic to hydrophilic after the introduction of amphiphilic HFBI. ATR-FTIR and XPS confirmed the presence of self-assembled HFBI films on the surface of the PCL nanofibers. The biocompatibility of the HFBI-modified PCL grafts was evaluated by cell proliferation in vitro, and by arteriovenous shunt (AV shunt) experiments ex vivo. Anti-CD31 antibody, which is specific for endothelial cells (ECs), was subsequently immobilized on the HFBI-coated PCL scaffolds through protein-protein interactions. This bioactive PCL graft was found to promote the attachment and retention of endothelial cells. These results suggest that this stepwise strategy for introducing cell-specific binding molecules into PCL scaffolds may have potential for development of vascular grafts that can endothelialize rapidly in vivo.
聚己内酯（PCL）接枝的亲水性改善和生物活性表面设计对于其在组织工程中的应用至关重要。在此，我们通过用类 II 疏水蛋白（HFBI）涂层修饰静电纺丝 PCL 接枝来实现稳定亲水表面的便捷方法。静态水接触角（WCA）表明，在引入两亲性 HFBI 后，PCL 接枝从疏水性转变为亲水性。ATR-FTIR 和 XPS 证实了自组装的 HFBI 薄膜存在于 PCL 纳米纤维的表面上。通过体外细胞增殖和体外动静脉分流（AV 分流）实验评估了 HFBI 修饰的 PCL 接枝的生物相容性。随后，通过蛋白-蛋白相互作用将特异性针对内皮细胞（ECs）的抗 CD31 抗体固定在 HFBI 涂覆的 PCL 支架上。发现这种生物活性 PCL 接枝可以促进内皮细胞的附着和保留。这些结果表明，将细胞特异性结合分子逐步引入 PCL 支架的策略可能具有开发能够在体内快速内皮化的血管移植物的潜力。
