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基于携带 sau1-hsdS1 的限制修饰系统，快速 PCR 检测金黄色葡萄球菌克隆群 398

Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1.

机构信息

Department of Microbiological Surveillance and Research, Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark.

出版信息

J Clin Microbiol. 2011 Feb;49(2):732-4. doi: 10.1128/JCM.01970-10. Epub 2010 Dec 1.

DOI:10.1128/JCM.01970-10
PMID:21123532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3043477/
Abstract

A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.

摘要

针对 Sau1-hsdS1 的 PCR 检测方法被开发出来，用于快速检测金黄色葡萄球菌克隆群 398（CC398）。通过对大量菌株（n=1307）的评估，该检测方法显示出高灵敏度（100%）和特异性（100%）。我们建议在医院和农场使用这种检测方法，以实现对耐甲氧西林金黄色葡萄球菌（MRSA）CC398 的准确、快速和廉价诊断。

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本文引用的文献

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Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398.耐甲氧西林和甲氧西林敏感金黄色葡萄球菌 398 型的短期微观进化和 PCR 检测。
Eur J Clin Microbiol Infect Dis. 2010 Jan;29(1):119-22. doi: 10.1007/s10096-009-0816-3. Epub 2009 Oct 2.
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Methicillin-resistant Staphylococcus aureus: a new zoonotic agent?耐甲氧西林金黄色葡萄球菌：一种新的人畜共患病原体？
Wien Klin Wochenschr. 2009;121(3-4):86-90. doi: 10.1007/s00508-008-1126-y.
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6
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