Bingen E, Lambert-Zechovsky N, Mariani-Kurkdjian P, Doit C, Aujard Y, Fournerie F, Mathieu H
Department of Microbiology, Hôpital Robert Debré, Paris, France.
Eur J Clin Microbiol Infect Dis. 1990 Apr;9(4):278-81. doi: 10.1007/BF01968060.
Eighty-five cerebrospinal fluid (CSF) specimens from the same number of pediatric patients with meningitis were examined to determine the bacterial count and the relationship of this count to the microscopy results, the ages of the patients and the bacterial species isolated. Bacterial counts ranged from 2 x 10 to 4 x 10(9) CFU/ml CSF. Twenty-five percent of the 85 CSF specimens positive for Haemophilus influenzae type b, Neisseria meningitidis, Streptococcus pneumoniae, Escherichia coli K1 and group B streptococci had counts of 10(7) CFU/ml or higher. Children between 1 and 6 months of age had significantly higher counts (p less than 0.05) than the other age groups. The three patients who had positive CSF cultures 24 h after the start of therapy all had initial bacterial counts of 10(7) CFU/ml or higher. The detection limit for Gram stain/microscopy was 10(5) CFU/ml. No correlation was found between bacterial count and the number of polymorphonuclear leukocytes.
对来自相同数量患脑膜炎儿科患者的85份脑脊液（CSF）标本进行检测，以确定细菌计数以及该计数与显微镜检查结果、患者年龄和分离出的细菌种类之间的关系。细菌计数范围为2×10至4×10⁹CFU/ml脑脊液。在85份检测出b型流感嗜血杆菌、脑膜炎奈瑟菌、肺炎链球菌、大肠埃希菌K1和B组链球菌呈阳性的CSF标本中，25%的标本细菌计数为10⁷CFU/ml或更高。1至6个月大的儿童的细菌计数显著高于其他年龄组（p<0.05）。治疗开始24小时后CSF培养呈阳性的3名患者，其初始细菌计数均为10⁷CFU/ml或更高。革兰氏染色/显微镜检查的检测限为10⁵CFU/ml。未发现细菌计数与多形核白细胞数量之间存在相关性。
