Tucker K D, Carrig P E, Wilkins T D
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
J Clin Microbiol. 1990 May;28(5):869-71. doi: 10.1128/jcm.28.5.869-871.1990.
Clostridium difficile is the cause of antibiotic-associated colitis in humans. The organism produces toxin A, which is generally known as the enterotoxin, and toxin B, which is known as the cytotoxin. Toxin A has been reported to have slight cytotoxic activity; in this study we show that cell lines (F9, OTF9-63, and P19) which express a carbohydrate to which toxin A binds are more sensitive to the toxin. These cell lines can be used as research tools for determining concentrations of biologically active toxin A and should also prove useful for studies of the mechanism of action of the toxin.
艰难梭菌是人类抗生素相关性结肠炎的病因。该菌产生毒素A（通常称为肠毒素）和毒素B（称为细胞毒素）。据报道，毒素A具有轻微的细胞毒性活性；在本研究中，我们表明表达毒素A所结合碳水化合物的细胞系（F9、OTF9 - 63和P19）对该毒素更为敏感。这些细胞系可用作测定生物活性毒素A浓度的研究工具，也应证明对毒素作用机制的研究有用。
