猿猴病毒40转化的非生产性细胞中游离病毒DNA的存在。
Presence of free viral DNA in simian virus 40-transformed nonproducer cells.
作者信息
Daya-Grosjean L, Monier R
出版信息
J Virol. 1978 Aug;27(2):307-12. doi: 10.1128/JVI.27.2.307-312.1978.
Abstract
Extracts from several simian virus 40 (SV40)-transformed nonproducer cells were prepared by the hot-phenol procedure normally used to extract cellular RNA. These extracts contained SV40 infectious units. Part of the infectious units were identified as SV40 form I DNA molecules. The results of reconstruction experiments suggest that SV40 form I DNA is extractable by the hot-phenol procedure because of its fast renaturation rate. The significance of the presence of free viral DNA in nonproducer transformed cells is discussed.
摘要
通过通常用于提取细胞RNA的热酚法制备了几种猿猴病毒40（SV40）转化的非生产性细胞的提取物。这些提取物含有SV40感染单位。部分感染单位被鉴定为SV40 I型DNA分子。重建实验结果表明，SV40 I型DNA因其快速复性率可通过热酚法提取。本文讨论了非生产性转化细胞中游离病毒DNA存在的意义。
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本文引用的文献
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Activation of infectious SV40 synthesis in transformed cells.转化细胞中感染性SV40合成的激活。
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Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells.猴病毒40的转录。I. 猴病毒40 DNA链的分离以及分离出的链与从裂解感染和转化细胞中提取的RNA的杂交。
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Demonstration of infectious deoxyribonucleic acid in transformed cells. I. Recovery of simian virus 40 from yielder and nonyielder transformed cells.转化细胞中传染性脱氧核糖核酸的证明。I. 从产生病毒和不产生病毒的转化细胞中回收猴病毒40
J Virol. 1972 Sep;10(3):399-409. doi: 10.1128/JVI.10.3.399-409.1972.
8
The integrated state of viral DNA in SV40-transformed cells.猿猴病毒40（SV40）转化细胞中病毒DNA的整合状态。
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288-95. doi: 10.1073/pnas.60.4.1288.
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[Study of the induction mechanism in Syrian hamster cells transformed by SV40 virus. I. Properties of a clonal cell line].[SV40病毒转化的叙利亚仓鼠细胞诱导机制的研究。I. 一个克隆细胞系的特性]
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Selective extraction of polyoma DNA from infected mouse cell cultures.从受感染的小鼠细胞培养物中选择性提取多瘤病毒DNA。
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