感染猿猴病毒40的仓鼠细胞中的病毒特异性脱氧核糖核酸：与S抗原和T抗原的相关性
Virus-specific deoxyribonucleic acid in simian virus 40-exposed hamster cells: correlation with S and T antigens.
作者信息
Levine A S, Oxman M N, Henry P H, Levin M J, Diamandopoulos G T, Enders J F
出版信息
J Virol. 1970 Aug;6(2):199-207. doi: 10.1128/JVI.6.2.199-207.1970.
Abstract
Several homologous hamster embryonic cell lines, transformed in association with simian virus (SV) 40 infection, were examined for the presence of deoxyribonucleic acid (DNA) complementary to SV40 ribonucleic acid (RNA) made in vitro. The methods employed permitted the detection of 10(-5) mug of viral DNA in 100 mug of cellular DNA, corresponding to one-fifth of an SV40 DNA molecule per cell. Those lines which contained both the SV40 surface (S) and tumor (T) antigens also contained DNA complementary to SV40 RNA synthesized in vitro. In contrast, neither of two lines which contained S, but not T, antigen contained detectable DNA complementary to SV40 RNA. These findings suggest that the production of S antigen does not depend upon the persistence of SV40 DNA in transformed cells.
摘要
对几种与猿猴病毒（SV）40感染相关而转化的同源仓鼠胚胎细胞系，检测了与体外合成的SV40核糖核酸（RNA）互补的脱氧核糖核酸（DNA）的存在情况。所采用的方法能够在100微克细胞DNA中检测到10^(-5)微克的病毒DNA，相当于每个细胞中有五分之一的SV40 DNA分子。那些同时含有SV40表面（S）抗原和肿瘤（T）抗原的细胞系也含有与体外合成的SV40 RNA互补的DNA。相比之下，含有S抗原但不含有T抗原的两个细胞系中，均未检测到与SV40 RNA互补的可检测DNA。这些发现表明，S抗原的产生并不取决于SV40 DNA在转化细胞中的持续存在。
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