ter Schegget J, Voves J, van Strien A, van der Noordaa J
J Virol. 1980 Aug;35(2):331-9. doi: 10.1128/JVI.35.2.331-339.1980.
The biological properties of nine clonal lines of BK virus-induced hamster tumor cells were studied. All clonal lines were oncogenic and showed an enhanced ability to form colonies in semisolid medium. The cells of each clonal line contained T antigen; no virus could be rescued from any of the clonal lines. The number of viral DNA copies was determined in three of the clonal lines and varied from 10 to 20 copies per diploid amount of cell DNA. The state of the viral genome was studied in these lines, and the great majority of the viral DNA molecules appeared to be present as free (nonintegrated) molecules. At least six length classes of free defective BK virus DNA molecules, which all lacked a part of the late region of the genome, were detected in these cells. Three of the six length classes of BK virus DNA molecules acquired a TaqI recognition site, which suggested substitution of cellular DNA.
对9个BK病毒诱导的仓鼠肿瘤细胞克隆系的生物学特性进行了研究。所有克隆系都具有致癌性，并且在半固体培养基中形成集落的能力增强。每个克隆系的细胞都含有T抗原；未从任何克隆系中拯救出病毒。在其中3个克隆系中测定了病毒DNA拷贝数，每二倍体细胞DNA中的病毒DNA拷贝数在10至20个之间。对这些克隆系中病毒基因组的状态进行了研究，绝大多数病毒DNA分子似乎以游离（非整合）分子的形式存在。在这些细胞中检测到至少6种长度类别的游离缺陷型BK病毒DNA分子，它们都缺少基因组晚期区域的一部分。6种长度类别的BK病毒DNA分子中有3种获得了TaqI识别位点，这表明有细胞DNA的替代。
