连续检测急性缺血性脑卒中患者的血浆核和线粒体 DNA 水平的价值。
The value of serial plasma nuclear and mitochondrial DNA levels in patients with acute ischemic stroke.
机构信息
Department of Neurology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan.
出版信息
Clin Chim Acta. 2011 Feb 20;412(5-6):476-9. doi: 10.1016/j.cca.2010.11.036. Epub 2010 Dec 3.
BACKGROUND
Elevated circulating cell-free DNA in plasma is reported in several critical diseases. This study hypothesized that since plasma nuclear and mitochondrial DNA substantially increase after acute ischemic stroke and decrease thereafter, their levels can predict treatment outcomes.
METHODS
Plasma nuclear and mitochondrial DNA levels were serially examined in 50 acute ischemic stroke patients and in 50 at risk control subjects during the study period.
RESULTS
Levels of plasma nuclear and mitochondrial DNA in patients with acute ischemic stroke were significantly higher than those in the controls (p<0.05). Elevated circulating nuclear DNA in plasma persisted until one month after the acute stroke. Levels of plasma nuclear DNA positively correlated to the clinical severity of stroke as reflected by the National Institutes of Health Stroke Scale.
CONCLUSION
Levels of plasma nuclear and mitochondrial DNA reflect the severity of cerebral damage after acute cerebral infarction. Assay of plasma DNA levels can be considered a neuro-pathologic marker of patients with acute ischemic stroke.
背景
已有研究报道，在多种危急病症中，血浆中的循环游离 DNA 水平会升高。本研究假设，由于急性缺血性脑卒中后，血浆核 DNA 和线粒体 DNA 含量大幅增加，随后又逐渐降低，因此其水平可以预测治疗效果。
方法
在研究期间，对 50 例急性缺血性脑卒中患者和 50 例高危对照者的血浆核 DNA 和线粒体 DNA 水平进行了连续检测。
结果
急性缺血性脑卒中患者的血浆核 DNA 和线粒体 DNA 水平显著高于对照组（p<0.05）。急性脑卒中后一个月内，血浆中循环核 DNA 的水平仍持续升高。血浆核 DNA 水平与国立卫生研究院卒中量表（NIHSS）所反映的脑卒中严重程度呈正相关。
结论
血浆核 DNA 和线粒体 DNA 水平反映了急性脑梗死后脑损伤的严重程度。检测血浆 DNA 水平可被视为急性缺血性脑卒中患者的神经病理标志物。
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