The CAS Key Laboratory of Engineering Plastics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
Colloids Surf B Biointerfaces. 2011 Jun 15;85(1):73-80. doi: 10.1016/j.colsurfb.2010.11.016. Epub 2010 Nov 17.
Poly(lactic acid) (PLA) microspheres have great potential in bone tissue engineering. However, their applications have been limited by surface and bulk properties such as hydrophobicity, lack of cell recognition sites and acidic degradation products. Apatite is a mineral which can effectively promote the adhesion and growth of bone cells. In this study, the bonelike mineral, carbonate apatite, was successfully used to functionalize porous PLA microspheres by a biomimetic mineralization method. To improve apatite formation, porous PLA microspheres were first selectively hydrolyzed in NaOH solution to increase the density of polar anionic groups on the surface, and then immersed in simulated body fluid for biomineralization. The morphology, composition, and phase structure of bioactive mineral grown on the original and hydrolyzed PLA microspheres were analyzed and compared quantitatively. The results showed that the hydrolysis which took place on the PLA microspheres enhanced the nucleation and growth of apatite. MG-63 cells attached well and spread actively on the mineralized PLA microspheres, indicating their strong potential in bone tissue engineering.
聚乳酸（PLA）微球在骨组织工程中有很大的应用潜力。然而，其表面和体相性质如疏水性、缺乏细胞识别位点和酸性降解产物限制了它们的应用。磷灰石是一种可以有效促进骨细胞黏附和生长的矿物质。在这项研究中，采用仿生矿化方法成功地用类骨矿物碳酸盐磷灰石对多孔 PLA 微球进行了功能化。为了促进磷灰石的形成，首先将多孔 PLA 微球在 NaOH 溶液中选择性水解，以增加表面极性阴离子基团的密度，然后将其浸入模拟体液中进行生物矿化。对原始和水解后的 PLA 微球上生长的生物活性矿物的形貌、组成和相结构进行了定量分析和比较。结果表明，PLA 微球上发生的水解增强了磷灰石的成核和生长。MG-63 细胞很好地附着在矿化的 PLA 微球上并积极扩展，表明其在骨组织工程中有很大的应用潜力。
