Chen Da, Ji Jian, Shen Jiacong
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2005 Oct;22(5):966-70.
Poly(lactic acid) microspheres with different surface charges have been prepared by using cationic, anionic or nonionic surfactants as the microspheres' surface stabilizers. Embedded with these microspheres, the modified PLA membranes with different surface charges have been obtained. The test of stability by CLSM and the morphological test by SEM confirmed that we obtained the microspheres modified PLA membranes with different surface charges successfully. The chondrocyte compatibility test of these modified PLA membranes showed that the attachment, proliferation and activity of chondrocytes on the positive surface of the modified PLA were better than those of other modified PLA membranes. The positive charge on the surface of PLA membrane could improve the cell-compatibility of PLA well.
通过使用阳离子、阴离子或非离子表面活性剂作为微球的表面稳定剂，制备了具有不同表面电荷的聚乳酸微球。嵌入这些微球后，获得了具有不同表面电荷的改性聚乳酸膜。通过共聚焦激光扫描显微镜（CLSM）进行的稳定性测试和通过扫描电子显微镜（SEM）进行的形态学测试证实，我们成功获得了具有不同表面电荷的微球改性聚乳酸膜。这些改性聚乳酸膜的软骨细胞相容性测试表明，软骨细胞在改性聚乳酸阳性表面上的附着、增殖和活性优于其他改性聚乳酸膜。聚乳酸膜表面的正电荷可以很好地改善聚乳酸的细胞相容性。
