Cooper Bret, Campbell Kimberly B, Feng Jian, Garrett Wesley M, Frederick Reid
Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA.
Mol Biosyst. 2011 Mar;7(3):773-83. doi: 10.1039/c0mb00171f. Epub 2010 Dec 3.
Soybean rust, caused by the fungus Phakopsora pachyrhizi, is an emerging threat to the US soybean crop. In an effort to identify proteins that contribute to disease resistance in soybean we compared a susceptible Williams 82 cultivar to a resistant Williams 82 inbred isoline harboring the Rpp1 resistance gene (R-gene). Approximately 4975 proteins from nuclear preparations of leaves were detected using a high-throughput liquid chromatography-mass spectrometry method. Many of these proteins have predicted nuclear localization signals, have homology to transcription factors and other nuclear regulatory proteins, and are phosphorylated. Statistics of summed spectral counts revealed sets of proteins with differential accumulation changes between susceptible and resistant plants. These protein accumulation changes were compared to previously reported gene expression changes and very little overlap was found. Thus, it appears that numerous proteins are post-translationally affected in the nucleus after infection. To our knowledge, this is the first indication of large-scale proteomic change in a plant nucleus after infection. Furthermore, the data reveal distinct proteins under control of Rpp1 and show that this disease resistance gene regulates nuclear protein accumulation. These regulated proteins likely influence broader defense responses, and these data may facilitate the development of plants with improved resistance.
由真菌大豆锈菌（Phakopsora pachyrhizi）引起的大豆锈病，对美国的大豆作物构成了新出现的威胁。为了鉴定有助于大豆抗病性的蛋白质，我们将感病的威廉姆斯82品种与携带Rpp1抗性基因（R基因）的抗病威廉姆斯82近交自交系进行了比较。使用高通量液相色谱 - 质谱法检测了来自叶片细胞核提取物中的约4975种蛋白质。这些蛋白质中的许多都具有预测的核定位信号，与转录因子和其他核调节蛋白具有同源性，并且被磷酸化。对总光谱计数的统计揭示了感病和抗病植物之间具有差异积累变化的蛋白质组。将这些蛋白质积累变化与先前报道的基因表达变化进行比较，发现重叠很少。因此，似乎在感染后细胞核中有大量蛋白质受到翻译后影响。据我们所知，这是感染后植物细胞核中大规模蛋白质组变化的首次迹象。此外，数据揭示了受Rpp1控制的不同蛋白质，并表明这种抗病基因调节核蛋白积累。这些受调节的蛋白质可能影响更广泛的防御反应，并且这些数据可能有助于培育具有更高抗性的植物。
