用于遗传性非息肉病性结直肠癌诊断的常见微卫星单核苷酸标记物。
Frequent MSI mononucleotide markers for diagnosis of hereditary nonpolyposis colorectal cancer.
作者信息
Haghighi Mahdi Montazer, Javadi Gholam Reza, Parivar Kazem, Milanizadeh Saman, Zali Narges, Fatemi Seyed Reza, Zali Mohammad Reza
机构信息
Science and Research Branch, Islamic Azad University (IAU), and Research Center for Gastroenterology and Liver Diseases, Taleghani Hospital, Tehran, Iran.
出版信息
Asian Pac J Cancer Prev. 2010;11(4):1033-5.
BACKGROUND
Failure in the DNA mismatch repair system is commonly accompanied by microsatellite instability and leads to colorectal cancer. The aim of this study was to find the most frequent of five mononucleotide markers in order to devise the simplest diagnostic strategy for identification of patients with hereditary nonpolyposis colorectal cancer (HNPCC) who were defined by defects in mismatch repair system.
MATERIALS AND METHODS
78 patients with colorectal cancer were recruited for this investigation. Five mononucleotide markers, NR-27, NR-21, NR-24, BAT-25 and BAT-26, were used as a pentaplex panel to determine MSI status.
RESULTS
Two out of five mononucleotide markers, NR-21 (25.6%) and BAT-25 (23.1%) showed more instability than the others.
CONCLUSION
In defining individuals with colorectal cancer, BAT25 and NR-21 may provide diagnostic assistance.
背景
DNA错配修复系统功能缺陷通常伴有微卫星不稳定性，并导致结直肠癌。本研究的目的是找出五个单核苷酸标记中最常见的标记，以便设计出最简单的诊断策略，用于识别因错配修复系统缺陷而定义的遗传性非息肉病性结直肠癌（HNPCC）患者。
材料与方法
招募78例结直肠癌患者进行本研究。使用五个单核苷酸标记NR-27、NR-21、NR-24、BAT-25和BAT-26作为五重检测板来确定微卫星不稳定性状态。
结果
五个单核苷酸标记中的两个，NR-21（25.6%）和BAT-25（23.1%），显示出比其他标记更高的不稳定性。
结论
在确定结直肠癌个体时，BAT25和NR-21可能提供诊断帮助。
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